Use of DNA-dependent RNA polymerase transcripts as reporter mole

Details Use of DNA-dependent RNA polymerase transcripts as reporter mole Use of DNA-dependent RNA polymerase transcripts as reporter mole

1994-03-08

1997-05-13

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 9121, 536 243, 536 2432, C12Q 168, C12P 1934, C07H 2104

Patent

active

056291531

ABSTRACT:
A polydeoxynucleotide construct is disclosed for use, in conjunction with a DNA-dependent RNA polymerase, as a signal amplifier in nucleic acid hybridization assays. The construct contains a recognition sequence for a target oligonucleotide, a promoter sequence for a DNA-dependent RNA polymerase, and a polymerase template. A method of use for this construct in hybridization assays is also disclosed. The method involves formation of a hybridization complex comprising the construct and the target sequence; addition of a polymerase which is specific for the promoter in the construct; and quantification of the resulting RNA transcripts.

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