Use of continuous/contiguous stacking hybridization as a diagnos

Details Use of continuous/contiguous stacking hybridization as a diagnos Use of continuous/contiguous stacking hybridization as a diagnos

1997-05-13

2000-07-18

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 911, 536 221, 536 2532, 536 266, C12Q 168, C12P 1934, C07H 2104, C07H 1904

Patent

active

060905492

ABSTRACT:
A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates. A method is also provided for determining the length of a repeat sequence in DNA or RNA, and also for determining the base sequence of unknown DNA or RNA.

REFERENCES:
patent: 5403708 (1995-04-01), Brennan et al.
Khrapko et al. A method for DNA sequencing by hybridization with oligonucleotide matrix. J. DNA Sequencing and Mapping, vol. 1, pp. 375-388, 1991.

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