Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-01-16
1999-06-01
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436 94, C12Q 168
Patent
active
059087455
ABSTRACT:
A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates.
REFERENCES:
patent: 5403708 (1995-04-01), Brennan
patent: 5627032 (1997-05-01), Ulanovsky
Khrapko, K.R. et al. "J. DNA Sequencing and Mapping, vol. 1 pp. 375-388. (1991, Harwood Academic Publishers, U.K.).
Barski Victor Evgenievich
Kirillov Eugene Vladislavovich
Lysov Yuri Petrovich
Mirzabekov Andrei Darievich
Parinov Sergei Valeryevich
Horlick Kenneth R.
University of Chicago
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