Use of chromatographic supports having immobilized flocculating

Details Use of chromatographic supports having immobilized flocculating Use of chromatographic supports having immobilized flocculating

1993-10-29

1995-09-05

Scheiner, Toni R.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

210656, 210661, 210723, 210807, 422 59, 436524, 436526, 436531, 436536, 436538, 436539, 436541, 436824, 530412, 530413, 530417, 530418, 530421, G01N 33544, G01N 33545

Patent

active

054478707

ABSTRACT:
Disclosed is a primary chromatographic support containing an immobilized flocculating agent for use as a separation media for a secondary support consisting of microparticles used in affinity separation and heterogeneous immunoassays. In a specific embodiment, a flocculating agent such as polyethyleneimine is immobilized on a chromatographic resin packed in a column. The column so formed is then used to trap microparticles having an affinity ligand or binder for the ligand affixed thereon. Such microparticles are used as a solid support for the affinity reaction involved in a variety of immunoassay formats.

REFERENCES:
patent: 4469630 (1984-09-01), Flashner
patent: 4606825 (1986-08-01), Crane et al.
patent: 4661408 (1987-04-01), Lau et al.
patent: 4721573 (1988-01-01), Ramsden et al.
patent: 4935147 (1990-06-01), Ullman et al.

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