Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-12-21
1994-10-25
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 79, 435 792, 436518, 422 56, 422 681, G01N 33531, G01N 33543, G01N 3091
Patent
active
053588525
ABSTRACT:
A specific binding immunoassay method for reducing the "hook" effect for the measurement of C-Reactive Protein has been discovered for both solution and dry analytical elements comprising contacting a liquid sample containing C-reactive protein in the presence of calcium ions with (a) a first antibody Ab1 specific for C-reactive protein, Ab1 being immobilized on a water-insoluble substrate and (b) a labeled, unbound second antibody Ab2 specific for C-reactive protein to obtain a water-insoluble complex of Ab1, ligand, and Ab2; (2) separating the water-insoluble complex from the liquid sample and unreacted Ab2; and (3) measuring either the amount of Ab2 associated with said water-insoluble complex or the amount of unreacted Ab2 as an indication of the amount of C-reactive protein in the sample.
REFERENCES:
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4751190 (1988-06-01), Chiapetta et al.
patent: 4780422 (1988-10-01), Mitani et al.
patent: 4781890 (1988-11-01), Arai et al.
patent: 4782014 (1988-11-01), Serban et al.
patent: 4839276 (1989-06-01), Adolfsen et al.
patent: 4902630 (1990-02-01), Bennett et al.
patent: 4913883 (1990-04-01), Imai et al.
patent: 5003065 (1991-03-01), Merritt et al.
patent: 5128270 (1992-07-01), Delacroix et al.
Coll "Development of a Fast Solid-Phase Enzyme Immunoassay For C-Reactive Protein" Rev. esp. Fisiol., 44(4) 359-368, 1988.
Catty Antibodies: A Practical Approach vol. 1, IRL Press, Oxford England (1988) p. 14.
"Specificity of C-Reactive Protein for Choline Phosphate Residues of Pheumococcal C-Polysaccharide (35323)" by John E. Volanakis and Melvin H. Kaplan; Department of Medicine, Case Western Reserve University School of Medicine and Metropolitan General Hospital, Cleveland, Ohio 44109; pp. 612-614.
"Comparison of the Secondary Structures and Binding Sites of C-Reactive Protein and the Phosphorylcholine-Binding Muring Myeloma Proteins.sup.1 " by N. Martin Young and Ross E. Williams; The Journal of Immunology, Copyright 1978 by The Williams & Wilkins Co.; vol. 121, No. 5, Nov. 1978; pp. 1893-1898.
"Demonstration of Calcium-Induced Conformational Chenge(s) in C-Reactive Protein by Using Monoclonal Antibodies" by J. Michael Kilpatrick, John F. Kearney and John E. Volanakis; Molecular Immunology, Copyright 1982 by Pergamon Press Ltd.; vol. 19, No. 9; pp. 1159-1165.
"Monoclonal Antibodies to the Calcium-Binding Region Peptide of Human C-Reactive Protein Alter its Conformation" by Steven J. Swanson, Michael C. Mullenix and Richard F. Mortensen; The Journal of Immunology, Copyright 1991 by The American Association of Immunologists; vol. 147, No. 7, Oct. 1, 1991; pp. 2248-2252.
"Identification and Partial Characterization of Multiple Native and Neoantigenic Epitopes of Human C-Reactive Protein by Using Monoclonal Antibodies.sup.1 " by Shan-Ching Ying, Henry Gewurz, Carol M. Kinoshita, Lawrence A. Potempa and Joan N. Siegel; The Journal of Immunology, Copyright 1989 by the American Association of Immunologists; vol. 143, No. 1, Jul. 1, 1989; pp. 221-228.
J58171-668-A, "High Sensitivity Multilayer Analytical Element"; Konishiroku Photo KK; Chem. Abst. C83-111610.
J52123-295, "Reagent for Determination of Carbon-Reactive Protein"; Eiken Kagaku KK; Chem. Abst.
J0 1171-567-A, "Calcium Phosphate Immuno-Adsorbent for Blood"; Advance KK; Chem. Abst.
Eastman Kodak Company
Everett John R.
Green Lora M.
Scheiner Toni R.
LandOfFree
Use of calcium in immunoassay for measurement of C-reactive prot does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Use of calcium in immunoassay for measurement of C-reactive prot, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of calcium in immunoassay for measurement of C-reactive prot will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-134666