Use of blocking protein with high pH extraction in method to det

Details Use of blocking protein with high pH extraction in method to det Use of blocking protein with high pH extraction in method to det

1991-10-08

1995-03-21

Bidwell, Carol E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435 794, 435961, 435962, 435975, G01N 33569

Patent

active

053994841

ABSTRACT:
A method has been developed for determining microorganisms associated with periodontal diseases which is highly sensitive and shows very low background and cross-reactivity among various closely related antigens. Antigen is extracted at relatively high pH, and either before or immediately after extraction, the antigen-containing specimen is mixed with a blocking composition having at least about 0.2 weight percent of a non-immunoreactive blocking protein. The pH of the resulting mixture is kept high when contacted with the antibodies specific to the antigen of interest. The compositions and components needed for the assay can be supplied in a diagnostic test kit.

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