Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1997-11-04
1999-11-30
Prats, Francisco
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 18, 435 97, 435 99, 435100, C12Q 154, C12Q 134, C12P 1912, C12P 1918
Patent
active
059940921
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL SCOPE OF THE INVENTION
The deficiency or low activity in intestinal lactase which result in an insufficient or even zero capacity for digesting lactose, is rare as regards a congenital metabolical error, though it is a common syndrome in human adults. With the greater part of mammels, an acute decrease of the lactase activity exists from the moment of weaning. In humans with ancestors who have depended on a substantial consumption of milk or lacteous products during a long time, this decrease is less frequent. On the other hand, the deficiency or low activity in intestinal lactase is quite frequent in babies who are nursing.
The present invention is comprised in the field of bloodless evaluation methods of intestinal lactase acitivity.
BACKGROUND OF THE INVENTION
The determination of the intestinal lactose activity is of importance in paedriatrics and gastroenterology and may be directly carried out, parting from a mucous sample, or indirectly, parting from the level of glucose in the blood or from the expired hydrogen, after the administration of a dose to the individual.
The direct determination has the disadvantage of constituting a complex and expensive method since it requires specific instruments and very specialized personal for the technique of extraction of the sample which must be submitted to subsequent analysis, apart from its being disagreeable nad not lacking in danger to the individual.
The indirect determination has the advantage of being a technique involving blood, which requires the extraction of blood by a specialied person before the sample may be analyzed, apart from being complex and susceptible to errors, due to the existance of glucose originating from the digestion of other food taken by the individual, and of endogenous glucose which might have been mobilized.
Other methods of determination of the intestinal lactase are based on the fact that specific disaccharides are, based on their affinity to the lactase, subsceptible of acting as substrate of the lactase and transform, by the action of the enzyme, into specific monosaccharides which are absorbed easily by the intestine and eliminated through urine.
Thus, the methods disclosed in Spanish Patentes ES-P-478590 and ES-P-482073, are based on the evaluation "in vivo" by means of the oral administration of 3-O methyl lactase and the analysis of the 3-O-methyl-D-glucose in urine. However, said methods present the disadvantage that they require the employment of a chromatographic procedure for the detection of 3-O-methyl-D-glucose in urine, which implies complex and expensive facilites and analysis equipments.
On the other hand, in Spanish Patent ES-P-9001680 discloses the preparation of disaccharide 4-O-.beta.-galactopyranosyl-D-xylose of formula (I) ##STR1## for the evaluation of the intestinal lactase activity. Said disaccharide is administrated orally, it acts as substrate of the intestinal lactase and decomposes, in the intestinal tract, in xylose and galactose, absorbing the xylose and eliminating through urine where it may be directly evaluated by means of a simple colorimetric method. The disaccharide of Spanish Patent ES-P-9001680, presents the disadvantage that it presents, in spite of having a quite similar structure to that of the lactase, an enhanced affinity to the lactose. This implies that only a part of the digested 4-O-.beta.-galactopyranosyl-D-xylose, is hydrolized based on the enzymatic activity of the lactase and, therefore, the non decomposed part in xylose and galacatose is eliminated with the feces. This implies a relatively substantial margin of error in view that the low or even non existant values of the xylose in the analyzed urine, may originate both from the deficient or zero intestinal lactase activity and from a deficient hydrolization of the disaccharide due to its lack of affinity to the lactase. In order to relieve said margin of errors, it is necessary that the individual digest a substantial quantity of disaccharides, which in turn may lead to intestinal problems such as diarrhea and
REFERENCES:
Rivera-Sagredo et al, Carb. Res. 228:129-135 (1992).
Lopez et al, Biotech. Letters. 13(10):705-710 (1991).
Gorin et al, Can. J. Chem. 42:2307-2317 (1964).
Toone et al, Tetrahedron 45(17):5397-5405 (1989).
Aragon Reyes Juan Jose
Canada Vicinay Francisco Javier
Fernandez-Mayoralas Alvarez Alfonso
Lopez Alvarez Rosa
Martin Lomas Manuel
Consejo Superior Investigaciones Cientificas
Prats Francisco
Universidad Autonoma
LandOfFree
Use of .beta.-D-galactopyranosyl-D-xyloses for the preparation o does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Use of .beta.-D-galactopyranosyl-D-xyloses for the preparation o, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of .beta.-D-galactopyranosyl-D-xyloses for the preparation o will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1670326