Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1990-09-17
1992-11-10
Wax, Robert A.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352401, 43524021, 4351721, 4353201, 4351723, 536 27, 935 55, 935 60, 935 70, C12N 506, C12N 1506, C12N 1515
Patent
active
051622220
ABSTRACT:
This invention details a novel and unique plasmid vector for the dual purposes of either producing transformed insect cell clones or recombinant baculoviruses. The transformed insect cell clones will continuously and permanently produce an efficiently processed desired foreign gene product. The recombinant baculoviruses will transiently express the desired foreign gene during immediate early phase of infection. This unique vector employs promoters from two different immediate early baculovirus genes along with the natural occurring polyhedrin promoter and gene. This unique combination creates a situation where the virus is highly infectious in vivo and resistant to inactivation in nature, because it is occlusion positive, and expresses the foreign gene product during immediate early phase of infection. Therefore this virus would be very effective as a delivery system of pesticides.
REFERENCES:
patent: 4745051 (1988-05-01), Smith et al.
patent: 4879236 (1989-11-01), Smith et al.
patent: 5017478 (1991-05-01), Cashion et al.
Blissard and Rohrmann, Virology 170:537-555 (1989).
Carson, et al., Virology, 162:444-451 (1988).
Granados and Federici (Editors), The Biology of Baculoviruses, vol. I, Biological Properties and Molecular Biology, Chapter 9.
Guarino and Summers, J. Virol. 57:563-571 (1986).
Guarino and Summers, J. Virol. 60(1):215-223, (1986).
Guarino, et al., J. Virol. 60(1):224-229 (1986).
Guarino and Summers, J. Virol. 61(7):2091-2099 (1987).
Summers, Proceeding of American Society, In Press (1990).
Webb and Summers, Technique-A Journal of Methods in Cell and Molecular Biology, 2(4):173-188, Aug., 1990.
IBI. Catalog. (86/87) pp. 79 & 81.
Guarino Linda A.
Jarvis Donald L.
Low Christopher S. F.
Wax Robert A.
LandOfFree
Use of baculovirus early promoters for expression of foreign gen does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Use of baculovirus early promoters for expression of foreign gen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of baculovirus early promoters for expression of foreign gen will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2291863