Use of baculovirus early promoters for expression of foreign gen

Details Use of baculovirus early promoters for expression of foreign gen Use of baculovirus early promoters for expression of foreign gen

1989-07-07

1991-12-31

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351723, 4353201, 4352401, 43524021, 4351721, 935 70, 935 60, 935 55, C12N 506, C12N 1506, C12N 1515

Patent

active

050772147

ABSTRACT:
The present invention provides an alternative strategy for baculovirus-mediated foreign gene expression: the promoters from immediate-early or delayed-early baculovirus viral genes were used to obtain the continuous expression of foreign genes in stably-transformed insect cells. The IE1 or the 30K promoters from Autographa californica nuclear polyhedrosis virus can drive the continuous expression of a foreign gene in insect cells. IE1-.beta.-galactosidase or 39K-.beta.-galactosidase constructs were used in combination with the IE1-neomycin resistan

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