Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using bacteria
Reexamination Certificate
2002-04-18
2004-10-19
Tate, Christopher R. (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using bacteria
C424S093470, C424S094620, C424S094640, C424S094650, C424S094660, C435S109000, C435S131000, C435S194000, C435S825000, C435S849000, C435S874000, C514S012200, C530S866000, C530S867000
Reexamination Certificate
active
06806070
ABSTRACT:
The invention relates to the use of extracts of bacteria from the family Pseudomonadaceae, as cosmetic agents for in particular combating ageing of the skin in humans.
It is known that ageing of the skin manifests itself, firstly, through a decrease in the number and a fragmentation of the elastic fibres of the dermis. Elastin becomes more sensitive to lysis by elastase and the deterioration of the elastin leads to disorganization of the elastic fibres. These phenomena result in a loss of the elasticity of the skin and in the formation of wrinkles.
Another manifestation of ageing of the skin is dryness of the skin, which becomes rough, with a loss of flexibility of the epidermis and a tendency towards desquamation. In the connective tissue of the skin of young individuals, the high content of hyaluronates, which are highly hydrophilic, promotes moisturization of the dermis, which is an essential element of skin tonicity. During ageing, the hyaluronate content, and therefore the water content, of the dermis greatly decreases, with the unfortunate consequences that the skin is flaccid and there is less diffusion of dermal water to the epidermis, which dries out. In addition, as a consequence of the decrease in the water content of the dermis, the circulation of metabolites, of ions and of oxygen is in particular impeded and, therefore, the metabolism of the dermal and epidermal cells slows down. The decrease in the hyaluronate content is linked to the activity of an enzyme, hyaluronidase, which cleaves the glycosidic bonds of hyaluronates. For this reason, this enzyme plays a very important role in ageing of the skin.
In addition, the drying out of the epidermis decreases the gaseous exchanges with the ambient atmosphere at the surface of the skin. This phenomenon of gaseous exchange, called cutaneous respiration, decreases with age.
Moreover, it is known that exposure to sunlight may cause an inflammatory reaction in skin tissue and that, after repeated and prolonged exposure to sunlight, in particular to UVA radiation, the skin eventually becomes dried out, excessively wrinkled and lacking in flexibility: this premature ageing of the skin is called “photoageing”.
It is therefore desirable to find new means in particular for protecting the skin against accelerated or premature ageing, and for more effectively protecting the skin against damage caused by exposure to sunlight, including against photoageing of the skin.
It has now been discovered that extracts of bacteria from the family Pseudomonadaceae, and in particular bacteria of the genus Pseudomonas, when applied to the skin, are in particular capable of improving moisturization of the skin and protecting the skin against certain harmful consequences of inflammatory reactions subsequent to exposure to ultraviolet radiation. More generally, they are capable of decreasing and/or delaying ageing of the skin, including photoageing of the skin.
These bacterial extracts have in particular the property of inhibiting lesions of the connective tissue of the skin subsequent in particular to UV exposure. These bacterial extracts in fact have the property of inhibiting elastase release in areas of inflammation, as shown in the experimental section hereinafter, and they also have the property of inhibiting elastase activity. More generally, these bacterial extracts, when applied to the skin, have anti-inflammatory and soothing properties, and improve the appearance of skin which exhibits a local inflammation or micro-inflammations, including after exposure to sunlight.
These bacterial extracts also have an inhibitory effect on hyaluronidase activity. Thus, they make it possible to prevent or treat dryness of the skin, including after exposure to sunlight and in cases of natural or premature ageing of the skin, and also in cases of photoageing. In addition, they improve skin tonicity by promoting moisturization of the dermis.
A subject of the invention is therefore the use of an extract of at least one bacterium from the family Pseudomonadaceae, as a cosmetic agent for combating natural or premature ageing of the skin, including photoageing, for improving the appearance and tonicity of dry skin, for preserving or improving skin elasticity, and/or for improving the appearance of skin which exhibits an inflammatory reaction, including after exposure to sunlight. In the present application, the expression “combating” ageing of the skin means preventing delaying, or even treating, ageing of the skin.
Among the bacteria which may be used according to the invention, mention may be made in particular of:
Pseudomonas vesicularis
, one of the types of which is the strain deposited at the ATCC under the No. 11426.
Pseudomonas maltophilia
, one of the types of which is the strain deposited at the ATCC under the No. 13637.
Pseudomonas maltophilia
is also called
Stenotrophomonas maltophilia.
In the present application, the expression “extracts of bacteria” or “bacterial extracts” denotes both the biomasses obtained after culturing the bacteria and the products obtained from these biomasses, in particular after purification and/or sterilization and/or fractionation. For example, the biomasses may optionally be at least partially dehydrated and/or ground. They may be sterilized, for example by heating. Of course, the invention extends to the use of extracts comprising any fraction of the biomass which has the same anti-ageing of the skin properties as the whole biomass, and in particular fractions which inhibit elastase secretion in areas of inflammation and/or which inhibit hyaluronidase activity. In the present application, the notion of extracts also encompasses derivatives obtained by chemical modification of certain functional groups (amines for example).
The method for preparing a bacterial extract used according to the invention comprises the steps consisting in culturing, in vitro, the bacteria according to known methods and then in collecting the biomass obtained.
Bacteria of the family Pseudomonadaceae are strictly aerobic Gram-negative bacteria. They grow on ordinary nutrient media, for example at temperatures of the order of 25 to 30° C.
To separate and isolate the biomass, various known methods, such as filtration or centrifugation, may be used. It is also possible to dry the biomass and concentrate it by dehydration, in particular by heating under reduced pressure (for example at a temperature of the order of approximately 80 to 120° C.) or by lyophilization.
The bacterial extracts may be used in the form of derivatives, for example of at least partially acylated derivatives. The acylation is carried out using a carboxylic acid anhydride or with a corresponding acid chloride. Use may be made, for example, of acetic anhydride or acetyl chloride. The acylation reaction is carried out such that at least some of the primary and secondary amine groups present in the bacterial biomass are acylated. The proportions of acylating agents and the acylation reaction conditions are easily determined by assaying, according to conventional methods, the primary and secondary amine groups before and after the acylation reaction.
The extracts of Pseudomonadaceae, or derivatives thereof, are introduced, as active ingredients, into compositions intended to be applied to the skin and/or to the scalp. These compositions exhibit good skin tolerance.
The invention therefore relates to a cosmetic composition comprising, as an active ingredient, an extract of at least one bacterium from the family Pseudomonadaceae, in combination with an excipient which is acceptable in cosmetology.
The excipients present in the composition of the invention are usual excipients. They are excipients which are compatible with use on the skin, on the scalp and/or on the hair.
In the compositions used according to the invention, the bacterial extracts are generally present in a proportion of 0.0005% to 5%, for example of 0.001% to 2%, and in particular of 0.01% to 2%, by weight of bacterial solids, relative to the: weight of the composition.
These compositions may contain the bact
Breton Lionel
Hilaire Pascal
Martin Richard
Pineau Nathalie
L'Oreal
Srivastava Kailash C.
Tate Christopher R.
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