Use of autologous promoters to express gene products in bordetel

Details Use of autologous promoters to express gene products in bordetel Use of autologous promoters to express gene products in bordetel

1993-01-14

1997-07-01

Caputa, Anthony C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 911, 435 914, 435 9141, 4351723, 4352523, 4353201, 424 934, 4242351, 4242401, 4242531, 4242541, 536 237, 935 10, 935 12, 935 29, 935 41, 935 56, 935 72, C12N 1564, C12N 1531, C12N 121, A61K 3910

Patent

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056437536

ABSTRACT:
Autologous promoters are used to effect expression of gene products in Bordetella strains. Hybrid Bordetella genes are constructed comprising a Bordetella gene, particularly one encoding a Bordetella antigen, fused at an ATG codon to a native but autologous Bordetella promoter or other Bordetella strain. Genes and promoters from B. pertussis are preferred. B. pertussis, containing the hybrid gene by insertion into the chromosome of the organism by homologous recombination at specific loci, effects expression of the protein for which the Bordetella gene codes at a production rate different from that achieved for the homologous gene. Specific strains and plasmids are described.

REFERENCES:
patent: 5085862 (1992-02-01), Klein et al.
Sambrook et al. Molecular Cloning: A Laboratory Manual 2nd Edition. Cold Spring Harbour Laboratory CSH(NY) 1989, Chapter 16 & 17.
Zealey et al. Fems Microbiology Letters 56:123-126 1988.
Makoff et al, Bio/Technology 1990, 8:1030-1033.
Nicosia et al, Infec. Immun. 1987; 55:963-967.
Burnette et al, Bio/Technology 1988; 6:699-706.
Romanos et al, Vaccine. vol. 9, Dec. 1991, pp. 901-906.
Burnette, Biotechnology, 1990, 8:1002.
Burnette 1991, Vaccine Research, vol. 1, eds. W. Koff et al.
Nicosia et al., Proc. Natl. Acad. Sci., U.S.A., 83, 4631, (1986);.
Loosmore et al., Nucl. Acids Res., 17, 8365, (1989);.
Relman et al., Proc. Natl. Acad. Sci., U.S.A., 86, 2637, (1989);.
Charles et al., Proc. Natl. Acad. Sci., U.S.A., 86, 3354, (1989);.
Sanger et al., Proc. Natl. Acad. Sci. U.S.A., 74, 5463, (1977);.
Zealey et al., Bio/Technology, 8, 1025, (1990);.
Stibitz et al., Gene, 50, 133, (1986);.
Yacoob and Zealey, Nucl. Acids Res. 16 1639, (1988);.
Imaizumi et al., Infect. Immun. 41, 1138, (1983);.
Loosmore et al., Infect. and Immun. 58, 3653 (1990);.
Zealey et al., Appl. Environ. Microbiol. 58, 208 (1992).

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