Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including freezing; composition therefor
Patent
1996-03-25
1999-04-27
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
Including freezing; composition therefor
435243, 435260, 435325, 435404, 5361231, C12N 100, C12N 104
Patent
active
058979875
ABSTRACT:
Methods and compositions for cryopreserving somatic cells are provided. In one embodiment, a cell cryopreservation medium is provided which includes an effective amount of arabinogalactan to maintain the viability of cells upon freezing, storage and thawing. The cells may be cooled or frozen during storage to a temperature about or below 4.degree. C., for example, to about -196.degree. C. In one preferred embodiment, ultrarefined arabinogalactan is provided in the cryopreservation medium, optionally in combination with a second cryopreservation agent, such as dimethyl sulfoxide. The medium can be used for the cryopreservation of a wide variety of different cell types from different sources. For example, mammalian cells, including porcine, canine, human, equine, rodent and bovine cells can be cryopreserved in the medium. The presence of arabinogalactan in the medium protects the viability of cells in the medium during the process of freezing, storage and thawing.
REFERENCES:
patent: 4004975 (1977-01-01), Lionetti et al.
patent: 4007087 (1977-02-01), Ericsson
patent: 4327177 (1982-04-01), Shrimpton
patent: 4605558 (1986-08-01), Shrimpton
patent: 5071741 (1991-12-01), Brockbank
patent: 5116969 (1992-05-01), Adams et al.
patent: 5405742 (1995-04-01), Taylor
patent: 5478576 (1995-12-01), Jung et al.
Bongso, et al., "Improved Sperm Concentration Motility, And Fertilization Rates Following Ficoll Treatment Of Sperm In A Human In Vitro Fertilization Program", Fertility And Sterility, 51(5):850-854 (1989).
Borel-Rinkes, et al., "Long-Term Functional Recovery Of Hepatocytes After Cryopreservation In A Three-Dimensional Culture Configuration", Cell Transplantation, 1:281-292 (1992).
Buchala, et al., "Polysaccharides In The Culture Medium Of Cotton Cells Cultured In Vitro", Food Hydrocolloids, 1(5/6):359-363 (1987).
Charak et al., "A Novel Approach To Immunomodulation Of Frozen Human Bone Marrow With Interleukin-2 For Clinical Application", Bone Marrow Transplantation, 11:147-154 (1993).
CRYO '95 Program, The Society for Cryobiology, University of Wisconsin Medical School, Continuing Medical Education, Department of Surgery, and University of Wisconsin-Extension, Jul. 6-11, 1995, Madison, Wisconsin.
Degnan, et al., "Arabinogalactan Utilization In Continuous Cultures Of Bifidobacterium Longum: Effect Of Co-Culture With Bacteroides Thetaiotaomicron", Anaerobe, 1:103-112 (1995).
Fahy, "Equations For Calculating Phase Diagram Information For The Ternary Systems NaCl-Dimethylsulfoxide-Water And NaCl-Glycerol-Water", Biophys, J., 32:837-850 (1980).
Fahy, "Cryoprotectant Toxicity And Cryoprotectant Reduction: In Search Of Molecular Mechanisms.sup.1 ", Cryobiology, 27:247-268 (1990).
Feng et al., "Improved Endothelial Viability Of heart Valves Cryopreserved By A New technique", Eur. J. Cardiothorac. Surg., 6:251-255 (1992).
Hagenah and Bohnke, "Corneal Crypreservation With Chondroitin Sulfate", Cryobiology, 30:396-406 (1993).
Hill, et al., "Use Of Arabinogalactan To Obtain Washed Murine Platelets Free Of Contaminating Plasma Proteins And Appropriate For Studies Of Function, Morphology, And Thromboiesis," J. Lab. Clin. Med., 111:73-83 (1988).
Holtz, et al., "A Simple Saccharide Extender For Cryopreservation Of Rainbow Trout (Oncorhynchus Mykiss) Sperm," Proc. Fourth Intern. Symp. Repr. Phys. Fish. (1991).
Klebe and Mancuso, "Identification Of New Cryoprotective Agents For Cultured Mammalian Cells", In Vitro, 19:167-170 (1983).
Lovelock, "The Haemolysis Of Human Red Blood-Cells by Freezing And Thawing", Biochem. Biophys. Acta, 10:414-446 (1953).
Maisse, "Comparaison de l'effet cryoprotecteur de differents gludies sur le sperme de truite arc-en-ciel (Oncorhynchus mykiss)," Aquat. Living Resour., 7:217-219 (1994).
Mazur, "Freezing Of Living Cells; Mechanisms And Implications", Am. J. Physiol., 247:C125-142 (1984).
Mosmann, "Rapid Colorimetric Assay For Cellular Growth And Survival: Application To Proliferation And Cytotoxicity Assays", J. Immunol. Methods., 65:55-63 (1983).
Parrish, et al., "Effect Of Bovine Sperm Separation By Either Swim-Up Or Percoll Method On Success Of In Vitro Fertilization And Early Embryonic Development", Theriogenology, 44:859-869 (1995).
Pegg, "Perfusion Of Rabbit Kidneys With Cryoprotective Agents", Cryobiology, 9:411-419 (1972).
Paltov, et al., "Freezing Of Ram Sperm In Media Containg Polysaccharides", Oytseyodstyo, 10:38-39 (1980) (Abstract).
Prescott, et al., "Larch Arabinogalactan For Hepatic Drug Delivery: Isolation And Characterization Of A 9 kDa Arabinogalactan Fragment," Carbohydrate Research, 278:113-128 (1995).
Shier and Olsen, "Isotonic Sucrose Improves Cryopreservation Of Culture Mannalian," In Vitro Cell Dev. Biol., 31:336-337, 1995).
Smith, et al., "Use Of Postseparation Sperm Parameters To Determine The Method Of Choice For Sperm Preparation For Assisted Reproductive Technology", Fertility And Sterility, 63(3):591-597 (1995).
Stout, "Larch Arabinogalactan" in Industrial Gums, R.L. Whistle Ed., Academic Press, New York, 307-310 (1959).
Swanson, et al., "Effect Of Percoll Wash On Sperm Motion Parameters And Subsequent Fertility In Intrauterine Insemination Cycles", J. Assisted Repro. And Genetics, 12(1):48-54 (1995).
Tanphaichitr, et al., "Egg-Penetration Ability And Structural Properties Of Human Sperm Prepared By Percoll-Gradient Centrifugation", Gamete Research, 20:67-81 (1988).
Ellington Joanna E.
Oliver Sylvia Adams
Advanced Reproduction Technologies Inc.
Ware Deborah K.
Wityshyn Michael G.
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