Liquid purification or separation – Processes – Treating by enzyme
Patent
1995-10-26
1999-03-16
Drodge, Joseph W.
Liquid purification or separation
Processes
Treating by enzyme
210634, 210639, 252364, 435183, 435267, 435816, C12N 900, B01D 1100
Patent
active
058825207
ABSTRACT:
Aqueous two phase systems are provided which permit the extractive separation of biological materials. In a preferred embodiment, an aqueous solution of arabinogalactan defines at least one phase, and an aqueous solution of a second solute, such as a polyether, defines at least one other phase. In one embodiment, an aqueous two phase system is provided in which one phase is defined by an aqueous solution containing predominantly ultrarefined arabinogalactan, and the other phase is defined by an aqueous solution containing predominantly a polymer such as a poly(ethylene glycol). Biological materials which can be extracted and separated from mixtures using the aqueous two phase systems include cells, organelles, macromolecules and organic molecules. The aqueous two phase systems can be used in wide range of different applications including in bioconversions for the production and separation of enzyme reaction products. The aqueous two phase systems can be used to extract a wide range of biological materials from aqueous mixtures inexpensively on a large scale, while maintaining the biological activity of the materials.
REFERENCES:
patent: 4094971 (1978-06-01), Chedid et al.
patent: 4390450 (1983-06-01), Gibson et al.
patent: 4579661 (1986-04-01), Gustafsson et al.
patent: 4743550 (1988-05-01), Ananthapadmanabhan et al.
patent: 4921796 (1990-05-01), Rozzell
patent: 4980065 (1990-12-01), Hsu
patent: 5041226 (1991-08-01), Shibata et al.
patent: 5078886 (1992-01-01), Hsu
patent: 5093254 (1992-03-01), Giuliano et al.
patent: 5116969 (1992-05-01), Adams et al.
patent: 5478576 (1995-12-01), Jung et al.
Albertsson, P., Partition of Cell Particles and Macromolecules, Third edition, John Wiley & Sons, Title Page and Table of Contents, 7 pgs. (1986).
Albertsson, P., et al., "Phase Diagrams," Methods In Enzymology, 228, 3-13 (1994).
Armbruster, F., "Use of Cyclohexane in the Production of Pure alpha- and beta-Cyclodextrins," Proceedings of the Fourth International Symposium on Cyclodextrins, Munich, West Germany, Apr. 20-22, 33-39 (1988).
Bamberger, S., et al., "Preparation of Phase Systems and Measurement of Their Physicochemical Properties," Partitioning in Aqueous Two-Phase Systems, Academic Press, Inc., Chapter 3, 85-130 (1985).
Bograceva, T., et al., "Uber die thermodynamische Vertraglichkeit von Gummiarabicum und makromolekularen Komponenten der Globulinfraktion der Backerhefe," Die Nahrung, 27, 735-740 (1983).
Bogracheva, T., et al., "Use of Polysaccharides to Remove Lipids from the Protein Globulin Fraction of Baker's Yeast," Carbohydrate Polymers, 2, 163-170 (1982).
Brooks, D., et al., "Preparation and Analysis of Two-Phase Systems," Methods In Enzymology, 228, 14-27 (1994).
Clark, A., et al., "Pollen-stigma interactions: Identification and characterization of surface components with recognition potential," Proc. Natl. Acad. Sci., 76, 3358-3362 (1979).
Delbourg, M., et al., "Effect of PEG and other Additives on Cyclodextrin Production by Bacillus macerans Cyclomaltodextrin-Glycosly-Transferase," Biotechnology Letters, 15, 157-162 (1993).
Ekman, K., et al., "Some Physicochemical Properties of Arabinogalactan from Western Larch (Larix occidentalis Nutt.)," Tappi, 45, 477-481 (1962).
Groman, E., et al., "Arabinogalactan for Hepatic Drug Delivery," Bioconjugate Chem., 5, 547-556 (1994).
Hagmar, B., "Liver Lectins, Adhesion Molecules in Experimental Metastasis," Bakteriol. Suppl., 25, 138-145 (1994).
Hammar, L., "Concentration of Biomaterials: Virus Concentration and Viral Protein Isolation," Methods In Enzymology, 228, 640-658 (1994).
Han, I., et al., "Production of Cyclodextrin from Raw Starch in the Agitated Bead Reaction System and its Reaction Mechanism," Kor. J. Appl. Microbiol. Biotechnol., 19, 163-170 (1991).
Hashimoto, H., et al., "Concentration of the Conversion Mixture Solution by Using Reverse Osmosis Membrane," J. Jpn. Soc. Starch Sci., 32, 307-311 (1985).
Hayashida, K., et al., "Enhancement of Enzymatic Production of Cyclodextrins by Adding Polyethylene Glycol or Polypropylene Glycol," Journal of Fermentation and Bioengineering, 73, 239-240 (1992).
Hayashida, K., et al., "Enzymatic Hydrolysis of Soluble Starch in a Polyethylene Glycol-Dextran Aqueous Two-Phase System," Journal of Fermentation and Bioengineering, 69, 240-243 (1990).
Hosono, K., et al, "Separation of yeast protoplasts from membrane ghosts using an aqueous two-phase system," Biochimica et Biophysica Acta, 855, 189-192 (1986).
Hosseini, J., et al., "Comparison of Two Separation Techniques for the Determination of Blood Mononuclear Cell Magnesium Content," Journal of the American College of Nutrition, 4, 361-368 (1983).
Johansson, G., "Affinity Partitioning in PEG-containing Two phase Systems, Section 5.2:System Parameters," Poly(Ethylen Glycol) Chemistry: Biotechnical and Biomedical Applications, J. Milton Harris, ed., Plenum Press, NY, pp. 75-84 (1992).
Johansson, G., "Uses of Poly(ethylene Glycol) with Charged or Hydrophobic Groups," Methods In Enzymology, 228, 64-75 (1994).
Kim, T., et al., "Enzymatic Production of Cyclodextrins from Milled Corn Starch in an Ultrafiltration Membrane Bioreactor," Biotechnol. and Bioeng., 41, 88-94 (1993).
Kobata, A., "Glycobiology: An Expanding Research Area in Carbohydrate Chemistry," Acc. Chem. Res., 26, 319-324 (1993).
Kohler, K., et al., "Uses of Fusions of .beta.-Galactosidase and Peptides to Proteins," Methods In Enzymology, 228, 627-640 (1994).
Kroner, K., et al., "Extractive Enzyme Recovery: Economic Considerations," Process Biochem., 19, 170-179 (1984).
Kroner, K., et al., "Evaluation of Crude Dextran as Phase-Forming Polymer for the Extraction of Enzymes in Aqueous Two-Phase Systems in Large Scale," Biotechnol. and Bioeng., 24, 1015-1045 (1982).
Larsson, M., et al., "Characterization of Aqueous Two-Phase Systems Based on Polydisperse Phase Forming Polymers: Enzymatic Hydrolysis of Starch in a PEG-Starch Aqueous Two-Phase System," Biotechnol. and Bioeng., 31, 979-983 (1988).
Larsson, M., et al., "Integration of Bioconversion and Downstream Processing: Starch Hydrolysis in an Aqueous Two-Phase System," Biotechnol. and Bioeng., 33, 758-766 (1989).
Lawrence, A., "Arabinogalactan," Natural Gums for Edible Purposes, Noyes Data Corporation, NJ, Title Page, Table of Contents, and pp. 1-16 (1976).
Layne, E., "Spectrophotometric and Turbidimetric Methods for Measuring Proteins," Methods in Enzymology, 3, 447-455 (1957).
Lee, Y., et al., "Enhancement of enzymatic production of cyclodextrins by organic solvents," Enzym. Microb. Technol., 13, 499-503 (1991).
Lee, R., et al., "New Synthetic Cluster Ligands for Galactose/N-Acetylgalactosamine-Specific Lectin of Mammalian Liver," Biochem., 23, 4255-4261 (1984).
Ling, T., et al., "Reppal PES--a Starch Derivative for Aqueous Two-Phase Systems," Carbohydr. Polymers, 11, 43-54 (1989).
Lowry, O., et al., "Protein Measurement with the Folin Phenol Reagent," J. Biol. Chem., 193, 265-275 (1951).
Majumdar, T., et al., "Cross-linked arabinogalactan: A new affinity matrix for the purification of Ricinus communis lectins," Experientia, 34, 979-980 (1978).
Mattiasson, B., "Bioconversions in Aqueous Two-Phase Systems: An Alternative to Conventional Immobilization," Methods in Enzymology, 137, 657-667 (1988).
Muchmore, A., et al., "Spontaneous Cytotoxicity by Human Peripheral Blood Monocytes: Inhibition by Monosaccharides and Oligosoaccharides," Immunobiol., 158, 191-206 (1981).
Nazareth, M., et al., "Studies on Larch Arabogalactan I," J. Pharm. Sci., 50, 560-563 (1961).
Nazareth, M., et al., "Studies on Larch Arabogalactan II," J. Pharm. Sci., 50, 564-567 (1961).
Nguyen, A., et al., "Applications of pullulan in aqueous two-phase systems for enzyme production, purification and utilization," Appl. Microbiol. Biotechnol., 27, 341-346 (1988).
Owens, H., "The Viscosities of Arabogalactan Solutions," J. Am. Chem. Soc., 62, 930-932 (1940).
Poly(Ethylene Glycol) Chemistry, J. Milton Harris, ed., Plenum Press, NY, Title Page, Table of Contents, Chapters 4-6, 57-102 (1992).
Rendleman, J., "Enhanced production of cyclomaltooctaose (.gamma.-cyclodextrin)
Manley-Harris Merilyn
Richards Geoffrey N.
Drodge Joseph W.
The University of Montana
LandOfFree
Use of arabinogalactan in aqueous two phase extractions does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Use of arabinogalactan in aqueous two phase extractions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of arabinogalactan in aqueous two phase extractions will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-813977