Use of an anti-microbial compound for disinfection

Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – With halogen – nitrogen – oxygen – or phosphorus containing...

Reexamination Certificate

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C510S253000, C510S362000, C510S365000, C510S367000, C510S375000, C510S378000, C510S398000, C510S434000, C510S463000, C510S477000

Reexamination Certificate

active

06613728

ABSTRACT:

TECHNICAL FIELD
The present invention relates to the use of a liquid disinfecting solution comprising an anti-microbial compound to disinfect various surfaces including inanimate surfaces such as hard surfaces like walls, tiles, floors, countertops, tables, glass, bathroom surfaces, kitchen surfaces. Advantageously the present compositions also deliver excellent cleaning performance, excellent shine.
BACKGROUND
Anti-microbial compositions contain materials which have the ability to disinfect. It is generally recognised that a disinfecting material greatly reduces or even eliminates the micro-organisms existing on a surface.
Although disinfecting compositions based on known disinfecting materials like bleach, quaternary ammonium compound, essential oil or the like, provide good immediate disinfecting properties, they do not maintain the disinfecting activity of the compositions for prolonged periods after their application to a hard-surface, to disinfect. Also consumers are looking for disinfecting compositions that on top of the disinfecting properties also provide excellent cleaning performance, especially on greasy soils while not leaving visible streak/filming on the surface upon drying.
It is thus an object of the present to provide not only effective disinfecting performance of any treated surface, with maximum efficiency, but also a greatly prolonged disinfecting action after application. Another object of the present invention is to provide compositions delivering on top of immediate and long lasting disinfecting properties, also excellent cleaning performance while further improving the shine onto the surface treated.
Advantageously effective immediate disinfecting performance as well as long lasting disinfecting performance is provided using a low total level of disinfecting materials.
An advantage of the present compositions is that they may be used to provide effective immediate and long lasting disinfection on any surfaces, even at high dilution levels, i.e., up to dilution levels of from 1:100 (composition:water).
Another advantage of the present invention is that immediate and long lasting disinfection is provided on a broad range of pure bacterial strains including Gram positive and Gram negative bacterial strains. In an preferred aspect of the present invention the combination of a peroxygen bleach and/or an anti-microbial essential oil or active thereof and an anti-microbial compound as described herein is particularly effective on Gram negative bacterial strains.
SUMMARY OF THE INVENTION
According to the present invention there is provided the use of a liquid disinfecting solution comprising an anti-microbial compound of low volatility to provide long lasting disinfection.
The present invention further encompasses a process for disinfecting a hard-surface wherein a liquid disinfecting composition is applied onto said surface.
The present invention also encompasses the use of said liquid disinfecting composition as defined herein, packaged in a spray dispenser, as well as a wipe impregnated with a liquid disinfecting composition as defined herein.
DETAILED DESCRIPTION OF THE INVENTION
Anti-microbial Compound
The present invention relates to the use of a liquid disinfecting comprising an anti-microbial compound of low volatility as an essential ingredient thereof. By anti-microbial compound, it is meant any known ingredient having the ability of reducing or even eliminating by killing the micro-organisms existing on a surface. In a preferred aspect of the present invention the liquid disinfecting solution is a composition comprising an anti-microbial compound and other optional ingredients examples of which are described later.
The anti-microbial compound selected for use in the present invention exhibit low volatility. The term low volatility as used herein, is defined as a compound that does not readily vaporise and thus remains on the surface to which it is applied. In a preferred aspect of the present invention the anti-microbial compound has a vapour pressure of less than 0.1 mmHg when measured at 20° C. using standard methods of calculating vapour pressure. In another preferred aspect, the anti-microbial compound is selected from the group consisting of citric acid, benzoic acid, benzophenone or mixtures thereof. The anti-microbial compound can be in solid, but is preferably in liquid form. Citric acid is a particularly preferred anti-microbial compound. Citric acid is commercially available as an aqueous solution from Jungbunzlauer under the trade name Citric acid®.
The anti-microbial compound is preferably present in an effective amount. By effective amount it is meant an amount sufficient to allow the anti-microbial compound to perform its action, i.e. to reduce the number of micro-organisms existing on a given surface. The effective amount differs depending on the anti-microbial compound. Typically, the compositions of the present invention comprise from 0.001% to 10% by weight of the total composition of an anti-microbial compound, preferably from 0.01% to 5% and more preferably from 0.1% to 3%.
Effective disinfecting performance is obtained with the compositions according to the present inventions on a variety of micro-organisms including Gram negative bacteria like
Pseudomonas aeroginosa, Escherichia coli
, Salmonella as well as on Gram positive bacteria like Enterococcus hirae,
Staphylococcus aureus
, present on infected surfaces, even if used in highly diluted conditions, e.g. up to a dilution level of 1:100 (composition:water).
The disinfecting properties of a composition according to the present invention may be measured by the bactericidal activity of said composition. A test method suitable to evaluate the bactericidal activity of a composition on infected surfaces is described in European Standard, prEN 1040, CEN/TC 216 N 78, dated Nov. 1995 issued by the European committee for standardisation, Brussels. European Standard, prEN 1040, CEN/TC 216 N 78, specifies a test method and requirements for the minimum bactericidal activity of a disinfecting composition. The compositions according to the present invention pass this test, even if used in highly diluted conditions. Other suitable methods are the AOAC Use-dilution method, AOAC Germicidal Spray method, AOAC Wipes method (US) and AFNOR T72-190 (Europe).
Long lasting disinfection properties of the compositions herein may be measured by the bactericidal activity of said compositions. A test method suitable to evaluate the long lasting bactericidal activity of a composition may be as follow: First, the surfaces (e.g. glass) to be tested are respectively treated with either a composition according to the present invention or a reference composition, e.g., a negative control composed of pure water (for example by spraying the composition directly on the surface or first spraying the composition on a sponge used to clean the surface or when the composition herein is executed in the form of wipe by wiping the surface therewith). After a variable time frame (e.g. 12-24 hours) each surface is respectively inoculated with bacteria (10
5-7
cfu/slide) cultured in for example TSB (Tryptone Soya Broth) and left typically from a few seconds to 2 hours before evaluating the remaining living bacteria. Then living bacteria (if any) are recovered from the surface (by re-suspending the bacteria into the neutralisation broth and plating them on agar) and incubated at appropriate temperature, e.g. 37° C. to let them grow typically over night. Finally, an evaluation of the residual antibacterial efficacy of the composition is made by comparing side by side the cultures and/or dilutions thereof (e.g. 10
−2
or 10
−1
) resulting from the surfaces treated with the compositions according to the present invention and the reference composition.
Optional Ingredients
The liquid disinfecting solution as described herein may in fact be a component of a disinfecting composition. A disinfecting composition may comprise a variety of ingredients for example an additional disinfecting material, surfactant, chelati

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