Use of a protease in the extraction of chlamydial, gonococcal an

Details Use of a protease in the extraction of chlamydial, gonococcal an Use of a protease in the extraction of chlamydial, gonococcal an

1988-10-07

1992-06-16

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 736, 435262, 435267, 435272, 436 17, 436510, C12Q 100, C12Q 170, G01N 33531

Patent

active

051224496

ABSTRACT:
An extraction method for lysing chlamydial, gonococcal or herpes organisms and extracting detectable antigen therefrom involves the use of a protease. In particular, the antigen can be effectively extracted from a biological specimen which contains whole blood or mucous using a protease. The extracted antigen can be effectively detected in an immunoassay involving antibodies directed to the antigen. The protease is novel to the process and is obtained from Bacillus subtilisin.

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Schmitt et al., "Surface-Exposed Antigenic Cleavage Fragments of N. gonorrhoeae Proteins IA and IB", Infection & Immunology, Dec. 1986, pp. 841-845, 1986.
Caldwell et al, Infect. & Immun., 44(2); pp. 306-314 (1984).
Mearns et al, J. Clin. Microbiol., 26 (2); pp. 1810-1813 (Sep. 1, 1988).
Stockman et al, J. Clin. Microbiol., 16(5), pp. 965-967 (1982).

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