Use of a mixture of conjugated and unconjugated solid phase bind

Details Use of a mixture of conjugated and unconjugated solid phase bind Use of a mixture of conjugated and unconjugated solid phase bind

1990-03-20

1992-11-17

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 793, 435 794, 435 795, 435 71, 436501, 436528, 436532, 436533, 436534, C12Q 100, G01N 3353, G01N 33566, G01N 33544

Patent

active

051642999

ABSTRACT:
In a solid phase homogeneous or heterogeneous assay for detection or quantitation of an analyte in a biological fluid, use of a combination of unconjugated binding reagent and carrier-conjugated binding reagent immobilized on the solid phase provides enhanced assay performance.

REFERENCES:
patent: 4818688 (1989-04-01), Adamich et al.
Skurrie et al., (1983) Enzyme Linked Immunosorbent Assay for Rubella . . . J. Clin. Microbiol. 17:738-743.
Geerligs et al. (1988) The influence of pH and ionic strength . . . J. Immuno Meth 106:239-244.
Jitsukawa et al., (1989) Increased coating efficiency of antigens . . . J Immuno Meth 116:251-257.
Yolken, R. H., Reviews of Infectious Disease, vol. 4, pp. 35-68, 1982.
McCullough, K. C., et al., Journal of Immunological Methods, vol. 82, pp. 91-100, 1985.
Geerligs, H. J., et al., Journal of Immunological Methods, vol. 106, pp. 239-240, 1988.
Brennand, D. M., Journal of Immunological Methods, vol. 93, pp. 9-14, 1986.

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