Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-04-10
2007-04-10
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S006120, C435S320100, C536S023100, C536S023400
Reexamination Certificate
active
10776330
ABSTRACT:
The invention relates to the use of a fluorescent protein chosen in particular from autofluorescent proteins,for the detection of the non-covalent interactions between a target protein labeled with the fluorescent protein and one of its ligands labeled with a label consisting:either of a molecule which is capable of absorbing the light emitted by the fluorescent protein,or of a fluorescent substance,this detection taking place by fluorescence energy transfer:between the fluorescent protein and the above-mentioned fluorescent substance, the fluorescent substance being such that either it is excitable at the emission wavelength of the fluorescent protein, or it emits at the excitation wavelength of the fluorescent protein, orbetween the fluorescent protein and the above-mentioned molecule which is capable of absorbing the light emitted by the fluorescent protein.
REFERENCES:
patent: 5342789 (1994-08-01), Chick et al.
patent: 5439797 (1995-08-01), Tsien et al.
patent: 5625048 (1997-04-01), Tsien et al.
patent: 0 552 108 (1993-07-01), None
patent: 91/01305 (1991-02-01), None
patent: 96/41166 (1996-12-01), None
patent: 97/28261 (1997-08-01), None
Atsushi Miyawaki et al., “Fluorescent Indicators for Ca2+Based onGreen Fluorescent Porteins and Calmodulin,” Letters to Nature, vol. 388, Aug. 1997, pp. 882-887.
Valerie A. Romoser et al., “Detection in Living Cells of Ca2+—dependent changes in the Fluorescene . . . ” The Journal of Biological Chemistry, vol. 272, No. 20, 1997, pp. 13270-13274.
Valentin N. Petushkov et al., “Direct Measurement of Excitation Transfer in the Protein Complex of Bacterial Luciferase . . . ” Biochemistry, vol. 35, No. 25, 1996, pp. 8413-8418.
Robi D. Mitra et al., “Fluorence Resonance Energy Transfer Between Blue-Emitting and Red-Shifted Excitation . . . ” Gene, vol. 173, No. 1, 1996, pp. 13-17.
Alix Philippe
Galzi Jean-Luc
Centre National de la Recherche Scientifique (C.N.R.S.)
Guzo David
Joike Michele K.
Young & Thompson
LandOfFree
Use of a fluorescent protein for detecting interaction... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Use of a fluorescent protein for detecting interaction..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of a fluorescent protein for detecting interaction... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3766594