Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1989-01-11
1992-06-16
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 692, 4352401, 4352402, 4353201, 536 27, 935 6, 935 34, 935 70, C12N 1511, C12N 1512, C12N 1585, C12P 2100
Patent
active
051224585
ABSTRACT:
The present invention relates to the use of the polyadenylation signal from the gene for bovine growth hormone to achieve a high level of expression of peptides in eukaryotic cells. Accordingly, the present invention provides a recombinant DNA compound comprising: a first nucleotide sequence which contains a promoter capable of initiating transcription of a gene; a second nucleotide sequence which encodes a gene for a polypeptide other than bovine growth hormone, including genomic and other intron containing forms thereof; a third nucleotide sequence which contains a bovine growth hormone polyadenylation signal; and a fourth nucleotide sequence which optionally contains a selectable marker. The first, second and third sequences are operably linked in sequence to form a functional genetic unit capable of being expressed. The fourth sequence, which is optionally provided, is useful for determining whether or not the DNA of the present invention has been incorporated into a living cell.
REFERENCES:
patent: 4399216 (1983-08-01), Axel et al.
patent: 4405712 (1983-09-01), Vande Woude et al.
Sasavage, N. L., et al., "Use of Oligodeoxynucleotide Primers to Determine Poly(adenylic acid) Adjacent . . . Bovine Growth Hormone Messenger Ribonucleic Acid", Biochemistry, vol. 19, pp. 1737-1743 (1980).
Fitzgerald, M., et al., "The Sequence 5'-AAUAAA-3' Forms Part of the Recognition . . . SV40 mRNAs", Cell, vol. 24, pp. 251-260 (Apr. 1981).
Woychik, R. P., et al., "Cloning & nucleotide sequencing of the bovine growth hormone gene", Nucleic Acids Research, vol. 10, No. 22, pp. 7197-7210 (1982).
Woychik, R. P., et al., "Requirement for the 3' flanking region of the bovine growth hormone gene for accurate polyadenylylation", Proc. Natl. Acad. Sci., USA, vol. 81, pp. 3944-3948 (Jul. 1984).
Rottman, F. M., et al., "Role of Post-Transcriptional mRNA Modification in the Maintenance of Eucaryotic mRNA Levels", Chem. Abstr. vol. 102, 1349g (1985) of GENE EXPRESSION, Alfred Benzon Symposium 19, pp. 279-290 (1984).
Pennica, D., et al., "Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli", Nature, vol. 301, pp. 214-221 (Jan. 20, 1983).
Southern, P. J. & P. Berg, "Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene Under Control of the SV40 Early Region Promoter", Jr. of Molecular & Applied Genetics, vol. 1, pp. 327-341 (1982).
Stinski, M. F., et al., "Organization and Expression of the Immediate Early Genes of Human Cytomegalovirus", Jr. of Virology, vol. 46, No. 1, pp. 1-14 (Apr. 1983).
Stenberg, R. M., et al., "Structural Analysis of the Major Immediate Early Gene of Human Cytomegalovirus", Jr. of Virology, vol. 49, No. 1, pp. 190-199 (Jan. 1984).
Thomsen, D. R., et al., "Promoter-regulatory region of the major immediate early gene of human cytomegalovirus", Prov. Natl. Acad. Sci. USA, vol. 81, pp. 659-663 (Feb. 1984).
Hsiung, N., et al., "Efficient Production of Hepatitis B Surface Antigen Using a Bovine Papilloma Virus-Metallothionein Vector", Jr. of Molecular & Applied Genetics, vol. 2, pp. 497-506 (1984).
Subramani, S., et al., "Expression of the Mouse Dihydrofolate Reductase Complementary Deoxyribonucleic Acid in Simian Virus 40 Vectors", Molecular & Cellular Biology, vol. 1, pp. 854-864 (Sep. 1981).
Rao, R. N. & S. G. Rogers, "Plasmid pKC7: A Vector Containing Ten Restriction Endonuclease Sites Suitable for Cloning DNA Segments", GENE, vol. 7, pp. 79-82 (1979).
Urlaub, G & L. A. Chasin, "Isolation of Chinese hamster cell mutants deflicient in dihydrofolate reductase activity", Proc. Natl. Acad. Sci. USA, vol. 77, No. 7, pp. 4216-4220 (Jul. 1980).
Graham, F. L., et al., "Transformation of Mammalian Cells with DNA Using the Calcium Technique", Introduction of Macromolecules Into Viable Mammalian Cells (publisher Alan R. Liss, Inc., New York, N.Y.), pp. 3-25.
Pfarr, D. S., et al., "Differential Effects of Polyadenylation Regions on Gene Expression in Mammalian Cells", DNA, vol. 5, No. 2, pp. 115-122 (1986).
Higgs, D. R., et al., ".alpha.-Thalassaemia caused by a polydenylation signal mutation", Nature, vol. 306, pp. 398-400 (Nov. 24, 1983).
Hunt, L. T. & M. O. Dayhoff "9-Hormones and Active Peptides", Atlas of Protein Sequence & Structure (ed. M. O. Dayhoff, Natl. Biomed. Res. Fnd., Washington, DC), vol. 5, Suppl. 2, pp. 113-139 (1976).
Lingappa, V. R., et al., "Nascent prehormones are intermediates in the biosynthesis of authentic bovine pituitary growth hormone and prolactin", Proc. Natl. Acad. Sci. USA, vol. 74, No. 6, pp. 2432-2436 (Jun. 1977).
Goodwin Edward C.
Palermo Daniel P.
Post Leonard E.
Rottman Fritz M.
Thomsen Darrell R.
Armitage Robert A.
DeLuca Mark
Martinell James
The Upjohn Company
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