Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1993-06-17
1996-05-21
Feisee, Lila
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
5303881, 5303888, 53038826, 5303911, 5303913, 43524027, C07K 1640, C07K 1618, C07K 1600, C12N 512
Patent
active
055191207
ABSTRACT:
A monoclonal or polyclonal antibody directed against urokinase plasminogen activator receptor (u-PAR), or a subsequence, analogue or glycosylation variant thereof. Antibodies are disclosed which react with free u-PAR or with complexes between u-PA and u-PAR and which are capable of 1) catching u-PAR in ELISA, or 2) detecting u-PAR, e.g. in blotting, or 3) in radioimmunoprecipitation assay precipitate purified u-PAR in intact or fragment form, or 4) is useful for immunohistochemical detection of u-PAR, e.g. in immunostaining of cancer cells, such as in tissue sections at the invasive front, or 5) inhibits the binding of pro-u-PA and active u-PA and thereby inhibits cell surface plasminogen activation. Methods are disclosed 1) for detecting or quantifying u-PAR, 2) for targeting a diagnostic to a cell containing a u-PAR on the surface, 3) for preventing or counteracting proteolytic activity in a mammal. Methods for for selecting a substance suitable for inhibiting u-PA/u-PAR interaction, for preventing or counteracting localized proteolytical activity in a mammal, for inhibiting the invasion and/or metastasis comprise the use of the antibodies and of nude mice inoculated with human cancer cells which are known to invade and/or metastasize in mice and having a distinct color, f.x. obtained by means of an enzyme and a chromogenic substrate for the enzyme, the color being different from the cells of the mouse.
REFERENCES:
patent: 4791068 (1988-12-01), Loskutoff et al.
Sevier, E. D. et al, Clin Chem, 27(11):1797-1806, 1981.
Behrendt, N. et al., "The Human Receptor for Urokinase Plasminogen Activator," The Journal of Biological Chemistry, 265(11):6453-6460 (Apr. 15, 1990).
Blasi, F., "A Surface Receptor for Urokinase Plasminogen Activator: a Link Between the Cytoskeleton and the Extracellular Matrix," Protoplasma 145:95-98 (1988).
Blasi, F. et al., "The Urokinase Receptor and Regulation of Cell Surface Plasminogen Activation," Cell Differentiation and Development, 32:247-254 (1990).
Fibbi, G. et al., "The 17500 Molecular Weight Region of the A Chain of Urokinase is Required for Interaction with a Specific Receptor in A431 Cells," Biological Abstracts 82(2):AB-355 (Abstract No. 12855).
Hearing, V. et al., "Modulation of Metastatic Potential by Cell Surface Urokinase of Murine Melanoma Cells," Biological Abstracts, 85(10):AB-645 (Abstract No. 102514).
Kirchheimer, J. et al., "Functional Inhibition of Endogenously Produced Urokinase Decreases Cell Proliferation in a Human Melanoma Cell Line," Proc. Natl. Acad. Sci. USA, 86:5424-5428 (Jul. 1989).
Lin, W.-Ch., et al., "Bacterial lacZ Gene as a Highly Sensitive Marker to Detect Micrometastasis Formation During Tumor Progression," Cancer Research, 50:2808-2817 (May 1, 1990).
Nolli, M. L., et al., "A Monoclonal Antibody that Recognizes the Receptor Binding Region of Human Urokinase Plasminogen Activator," Thrombosis and Haemostasis, 56(2):214-218 (1986).
Ossowski, L. "In Vivo Invasion of Modified Chorioallantoic Membrane by Tumor Cells: the Role of Cell Surface-bound Urokinase" The Journal of Cell Biology, 107(6):2437-2445 (Dec. 1988).
Roldan, A. L., et al., "Cloning and Expression of the Receptor for Human Urokinase Plasminogen Activator, a Central Molecule in Cell Surface, Plasmin Dependent Proteolysis," The EMBO Journal, 9(2):467-474 (1990).
Ronne, E. et al., "Cell-induced Potentiation of the Plasminogen Activation System is Abolished by a Monoclonal Antibody the Recognizes the NH2-Terminal Domain of the Urokinase Receptor," FEBS Letters, 288(1,2):233-236 (Aug. 1991).
Appella, E., et al., "The Receptor-binding Sequence of Urokinase," J. Biol Chem., 262(10):4437-4440 (1987).
Bajpai, A., et al., "Cryptic Urokinase Binding Sites on Human Foreskin Fibroblasts," Biochem. Biophys. Res. Commun. 133(2):475-482 (1985).
Blasi, F., "Surface Receptors for Urokinase Plasminogen Activator," Fibrinolysis, 2:73-84 (1988).
Blasi, F., et al., "The Receptor for Urokinase-Plasminogen Activator," J. Cell. Biochem., 32:179-186 (1986).
Blasi, F., et al., "Urokinase-Type Plasminogen Activator: Proenzyme, Receptor, and Inhibitors," J. Cell. Biol., 104:801-804 (1987).
Boyd, D., et al., "Determination of the Levels of Urokinase and Its Receptor in Human Colon Carcinoma Cell Lines," Cancer Research, 48:3112-3116 (Jun. 1, 1988).
Boyd, D., et al., "Modulation of the urokinase receptor in human colon cell lines by N,N-dimethylformamide," Biochimica et Biophysica Acta, 947:96-100 (1988).
Burtin, P., et al., "Receptor for Plasmin on Human Carcinoma Cells," J. Nat'l. Cancer Inst., 80(10):762-765 (Jul. 20, 1988).
Cubeilis, M. V., et al., "Binding of Single-chain Prourokinase to the Urokinase Receptor of Human U937 Cells," J. Biol. Chem. 261(34):15819-15822 (Dec. 1986).
Dano, K., et al., "Plasminogen Activators, Tissue Degradation, and Cancer," Advances in Cancer Research, 41:140-264, (1985).
Dano, K., et al., "Plasminogen Activators and Neoplasia," Tissue-Type Plasminogen Activator (t-PA): Physiological and Clinical Aspects, Kluft, ed., CRC Press, Boca Raton, pp. 19-46 (1988).
Ellis, V., et al. "Role of human U937 monocytes in controlling single-chain urokinase-initiated plasminogen activation," Fibrinolysis, 2:112-114 (1988).
Ellis, V., et al., "Plasminogen Activation Initiated by a Single-Chain Urokinase-ytpe Plasminogen Activator," J. Biol. Chem., 264:2185-2188, (1988).
Estreicher, A., et al., "Characterization of the Cellular Binding Site for the Urokinase-Type Plasminogen Activator," J. Biol. Chem., 264(2):1180-1189, (Jan. 1989).
Hearing, V. J., et al., "Modulation of Metastatic Potential by Cell Surface Urokinase of Murine Melanoma Cells," Cancer Research, 48:1270-1278, (Mar., 1988).
Hebert, C. A., et al., "Linkage of Extracellular Plasminogen Activator to the Fibroblast Cystoskeleton: Colocalization of Cell Surface Urokinase with Vinculin," J. Cell Biol., 106:1241-1247, (Apr., 1987).
Mignatti, P., et al., "Tumor Invasion through the Human Amniotic Membrane: Requirement for a Proteinase Cascade," Cell, 47:487-498 (Nov. 1986).
Miles, L. A., et al., "Receptor Mediated Binding of the Fibrinolytic Components, Plasminogen and Urokinase, to Peripheral Blood Cells," Thrombosis and Haemostasis, 58(3):936-942 (1987).
Needham, G. K., et al., "Binding of urokinase to specific sites on human breast cancer membranes," Br. J. Cancer, 55:13-16 (1987).
Nelles, L., et al., "Characterization of Recombinant Human Single Chain Urokinase-type Plasminogen Activator Mutants Produced by Site-specific Mutagenesis of Lysine 158," J. Biol. Chem., 262(12):5682-89, (1987).
Nielsen, L. S., et al., "A 55,000-60,000 M.sub.r Receptor Protein for Urokinase-type Plasminogen Activator," J. Biol. Chem., 263(5):2358-2363, (Feb. 1988).
Ossowski, L., "Plasminogen Activator Dependent Pathways in the Dissemination of Human Tumor Cells in the Chick Embryo," Cell, 52:321-328 (Feb. 1988).
Ossowski, L., et al., "Antibodies to Plasminogen Activator Inhibit Human Tumor Metastasis," Cell, 35:611-619 (Dec. 1983).
Pannell, R., et al., "Activation of Plasminogen by Single-Chain Urokinase or by Two-Chain Urokinase--A Demonstration That Single-Chain Urokinase Has A Low . . . ," Blood, 69(1):22-26 (Jan. 1987).
Petersen, L. C., et al., "One-Chain Urokinase-type Plasminogen Activator from Human Sarcoma Cells Is a Proenzyme with Little or No Intrinsic Activity," J. Biol. Chem., 263(23):11189-11195, (Aug. 1988).
Picone, R., et al., "Regulation of Urokinase Receptors in Monocyte-like U937 Cells by Phorbol Ester PMA," J. Cell. Biol., 1989.
Plow, E. F., et al., "The Plasminogen System and Cell Surfaces: Evidence for Plasminogen and Urokinase Receptors on the Same Cell Type," J. Cell. Biol., 103(6):2411-2420 (Dec. 1986).
Pollanen, J., et al., "Ultrastructural Localization of Plasma Membrane-associated Urokinase-type Plasminogen Activator at Focal Contacts," J. Cell. Biol., 106:87-95, (Jan. 1988).
Pollanen, J., et al., "Distinct Localizations of Urokinase-type Plasminogen Activator and Its Type 1 Inhibitor under Cultured Human Fibroblasts and Sarcoma Cells," J. Cell. Biol., 104:1085-1096, (Apr., 1987).
Reich, R., et al., "Effect
Behrendt Niels
Bruenner Nils
Dano Keld
Ellis Vincent
Hoyer-Hansen Gunilla
Cancerforskningsfondet af 1989
Cooper Iver P.
Feisee Lila
Loring Susan A.
LandOfFree
Urokinase-type plasminogen activator receptor antibodies does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Urokinase-type plasminogen activator receptor antibodies, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Urokinase-type plasminogen activator receptor antibodies will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2038988