Urinary tract tissue graft compositions and methods for...

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Animal or plant cell

Reexamination Certificate

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C424S423000, C435S373000, C435S395000

Reexamination Certificate

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07344712

ABSTRACT:
A method for providing a urinary tract tissue graft composition includes providing a segment of small intestinal submucosa and positioning the segment of small intestinal submucosa in a tissue culture frame such that the segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame. At least one multipotent cell type is isolated from a tissue specimen of a subject and cultured, and then seeded upon a surface of the segment of small intestinal submucosa, thereby forming a urinary tract tissue graft. Methods for repairing a damaged urinary tract tissue of a subject are also disclosed.

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patent: 6576265 (2003-06-01), Spievack
patent: 2002/0087214 (2002-07-01), Kropp et al.
patent: 2002/0155098 (2002-10-01), Chancellor et al.
patent: 2003/0216811 (2003-11-01), Badylak
Lu et al.; “Muscle-Derived Stem Cells Seeded into Acellular Scaffolds Develop Calcium-Dependent Contractile Activity That Is Modulated By Nicotinic Receptors”; Urology 61:1285-1291, 2003.
Zhang et al; “‘Co-Culture’ of Bladder Smooth Muscle And Urothelial Cells On Small Intestinal Submococa (sis): Evaluation Of The Best Culture Method For in Vitro Tissue Engineering Techniques”; Pediatrics Journal Supp: Sep. 1999:807-808.
Zhang, Y. et al.; “Coculture of Bladder Urothelila and Smooth Muscle Cells on Small Intestinal Submucosa: Potential Applications for Tissue Engineering Technology”; Journal of Urology 164:928-935 (2000).

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