Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-07-23
1995-08-15
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 6, 435 711, 4351723, 4353201, 435 91, 536 231, 536 235, 536 243, 536 2431, C12P 2106
Patent
active
054418755
ABSTRACT:
An isolated nucleotide sequence encoding a urea transporter polypeptide or unique fragments of urea transporter polypeptide, is provided. One embodiment is an isolated DNA sequence (SEQ ID NO.:1) encoding a urea transporter polypeptide, that has at least two extended hydrophobic domains, each domain lacking interspersed hydrophilic domains. Also described are recombinant cells and plasmids containing the foregoing isolated DNA, preferably linked to a promoter. Isolated urea transporter polypeptide is provided, having at least two extended hydrophobic domains, each domain lacking interspersed hydrophilic domains (SEQ ID NO.:2). Portions of the foregoing isolated urea transporter polypeptides are also described. Antibodies with selective binding specificity for the polypeptides of the invention also are provided.
Methods for producing urea transporter polypeptide as well as methods for testing for modulators of urea transporter polypeptide activity are also described.
REFERENCES:
R. Zhang, et al., "Urea Transport in Freshly Isolated and Cultured Cells from Rat Inner Medullary Collecting Duct", 1990, J. Membrane Biol., vol. 117, pp. 253-261.
M. Hediger, et al., "Expression Cloning and cDNA Sequencing of the Na+/glucose Co-Transporter", Nov. 1987, Nature, vol. 330, No. 26, pp. 379-381.
M. Hediger, et al., "Expression of Size-Selected mRNA Encoding the Intestinal Na/glucose Cotransporter in Xenopus Laevis Oocytes", May 1987, Proc. Natl. Acad. Sci. USA, vol. 84, pp. 2634-2637.
Chou, C-L, et al., "Inhibition of Urea Transport in Inner Medullary Collecting Duct by Phloretin and Urea Analogues", May 1989, Natl. Heart, Lung, and Blood Inst., Bethesda, Md., pp. F359-F365.
R. Zhang, et al., "Water and Urea Permeability Properties of Xenopus Oocytes: Expression of mRNA from Toad Urinary Bladder"; 1991, Amer. Physiological Society, pp. C26-C34.
Brigham and Women's Hospital
Nucker Christine M.
Scheiner Laurie
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