Urea determination method

Details Urea determination method Urea determination method

1981-07-09

1985-03-26

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

C12Q 158

Patent

active

045073880

ABSTRACT:
In a method for the enzymic-colorimetric determination of urea in clinical analyses, the improvement consisting in that the sample to be tested is admixed with a composite reagent composed by urease, a buffer, an alkali-metal salicylate and a nitroprusside and, after a short incubation, a hypochlorite is added, a short additional incubation is performed and the color is measured photometrically. By this method, two reagent systems are sufficient instead of the usual three.

REFERENCES:
patent: 3119751 (1964-01-01), Chaney
patent: 3432395 (1969-03-01), Reardon
patent: 3542649 (1970-11-01), Searcy
patent: 3592741 (1971-07-01), Hughes
patent: 3769172 (1973-10-01), Bressler et al.
patent: 3876502 (1975-04-01), Monte et al.

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