Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-07
1999-04-06
Crouch, Deborah
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 701, 4352523, 435325, 435419, 536 241, C12P 2106, C12P 2104, C12N 500, C12N 120
Patent
active
058916652
ABSTRACT:
It has been shown that a 5' untranslated leader sequences of RNA virus non-structural genes act as enhancers of translation of mRNA. These leader sequences can be ligated upstream of an appropriate mRNA or used in the form of a cDNA expression vector. The translational enhancers are effective in many different types of expression systems, such as plant cells, bacterial cells and animal cells as well as translation systems made from these cells. Tobacco mosaic virus (TMV), turnip yellow mosaic virus (TYMV), brome mosaic virus (BMV), alfalfa mosaic virus (A1MV) and rous sarcoma virus (RSV) are examples of RNA viruses whose 5' untranslated leader sequences of non-structural genes enhance translation.
REFERENCES:
patent: 4820639 (1989-04-01), Gehrke
patent: 5466788 (1995-11-01), Ahlquist et al.
Gehrke, "Enhancing the Translation Efficiency of Plant Messenger RNAs", Abstract No. PO-1-147 in The Book of Abstracts from the First International Congress of Plant Molecular Biology, Savannah, 27th Oct.-1st Nov. 1985.
Lewin, Genes II, 2nd Edition, 1985, John Wiley & Son, NY, p. 165.
Pelletier et al, "Insertion Mutagenesis to Increase Secondary Structure Within the 5' Noncoding Region of a Eukaryotic mRNA Reduces Translational Efficiency," Cell, vol. 40,515-526, Mar. 1985.
Ravelonandro et al, "Structure of the 5'-Terminal Untranslated Region of the Genomic RNAs from Two Strains of Alfalfa Mosaic Virus", Nucleic Acids Research, vol. 11, No. 9, pp. 2815-2826 (1983).
Brederode et al, "Complete Nucleotide Sequence of Alfalfa Mosaic Virus RNA 4", Nucleic Acids Research, vol. 8, No. 10, pp. 2213-2223 (1980).
Crouch Deborah
Diatech Limited
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