Unmodified recombinant human adult hemoglobin production

Details Unmodified recombinant human adult hemoglobin production Unmodified recombinant human adult hemoglobin production

1994-08-30

1998-05-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 530385, C12P 2102, C12N 1563, C07K 14805

Patent

active

057534659

ABSTRACT:
Methods for obtaining unmodified recombinant human normal adult hemoglobin involve a novel expression plasmid that coexpresses human .alpha.- and .beta.-globin genes and E. coli methionine aminopeptidase genes under the control of separate tac promoters. Methods are also provided for correcting an abnormal conformation of some of the heme groups incorporated in the proteins expressed by the expression plasmid.

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