Universal targets for species identification

Details Universal targets for species identification Universal targets for species identification

1997-01-05

1999-11-23

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 911, 435 912, 536 231, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

059898216

ABSTRACT:
The present invention provides oligonucleotide primers and a method of using these primers for identification of the species of an organism, wherein the identification includes amplification of a variable polynucleotide sequence encoding a highly conserved region of a heat shock polypeptide.

REFERENCES:
patent: 5232833 (1993-08-01), Sanders et al.
patent: 5464750 (1995-11-01), Sanders et al.
patent: 5494796 (1996-02-01), Spears et al.
Perkin Elmer Cetus Geneamp DNA amplification reagent kit instructions (1990).
Sipos et al., "Cloning and sequencing of the gene coding for the 10-and 60-kDa heat shock proteins from Pseudomonas aeruginosa and mapping of a species specific epitope", Inf. Immun. 59(9):3219-3226 (1991).
Ohta et al., "Molecular characterization of the gene operon of heat shock proteins HSP60 and HSP10 in methicillin resistant staphlococcus aureus", Biochem. Biophys. Res. Comm. 193(2):730-737 (1993).
Hemmingsen et al., "Homologous plant and bacterial proteins chaperone oligomeric protein assembly", Nature 333:330-334 (1988).
Plikaytis et al., "Differentiation of Slowly Growing Myobacterium Species, Including Myobacterium tuberculosis, by Gene Amplification and Restriction Fragment Length Polymorphism Analysis", Journal of Clinical Microbiology, vol. 30, No. 7, pp. 1815-1822 (Jul. 1, 1992).
Telenti et al., "Rapid Identification of Mycobacterium to the Species Level by Polymerase Chain Reaction and Restriction Enzyme Analysis", Journal of Clinical Microbiology, vol. 31, No. 2, pp. 175-178, Feb. 1, 1993.
Octavian Lungu et al., "Differentiation of Nocardia from Rapidly Growing Mycobacterium Species by PCR-RFLP Analysis", Diagn. Microbiol. Infect. Dis., vol. 18, pp. 13-18, Jan. 18, 1994.
Goh et al., "HSP60 Gene Sequences as Universal Targets for Microbial Species Identification Studies with Coagulase-Negative Staphylococci", Journal of Clinical Microbiology, vol. 34, No. 4, pp. 818-823, Apr. 1996.

Affiliated with

Also associated with

Rating

Universal targets for species identification does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Universal targets for species identification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Universal targets for species identification will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1220158