Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-11-28
2006-11-28
Benzion, Gary (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S006120, C536S023100
Reexamination Certificate
active
07141372
ABSTRACT:
The invention relates to a novel Universal RT-coupled PCR strategy for the specific detection and accurate quantitation of mRNA. Claimed and disclosed are novel Universal reverse transcription (RT) primers, a specific primer mix containing the Universal RT-primers, a transcript specific forward primer and a reverse PCR primer identical to a unique tag sequence, and methods and kits thereof for avoiding the amplification of genomic DNA and/or pseudogenes.
REFERENCES:
patent: 5262311 (1993-11-01), Pardee et al.
patent: 5845267 (1998-12-01), Ronen
patent: 6777180 (2004-08-01), Fisher et al.
Ayala, M. et al. New Primer Strategy Improves Precision of Differential Display. 1995, BioTechniques, vol. 18, No. 5, pp. 842-844, 846, 848, 850.
Hurteau Gregory J.
Spivack Simmon D.
Benzion Gary
Frommer & Lawrence & Haug LLP
Health Research Incorporated
Kowalski Thomas J.
Woolwine Samuel C.
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