Universal restriction endonuclease

Details Universal restriction endonuclease Universal restriction endonuclease

1986-02-05

1990-06-19

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435199, 935 19, C12P 1934, C12N 922

Patent

active

049353578

ABSTRACT:
A universal restriction endonuclease for cleaving a target DNA at a predetermined site. The universal restriction endonuclease utilizes a tailored oligodeoxynucleotide adapter in conjunction with a restriction enzyme which cuts DNA at a known distance from its recognition site. The oligodeoxynucleotide adapter mimics the relationship which exists in such enzymes between its recognition site and cut site.

REFERENCES:
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4670379 (1987-06-01), Miller
Maniakis, T. et al., Moleendar Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, 1982, pp. 392-393.

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