Universal process for isolating and purifying nucleic acids from

Details Universal process for isolating and purifying nucleic acids from Universal process for isolating and purifying nucleic acids from

1996-12-16

2000-03-14

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 254, 435 411, C07H 106, C12N 1510

Patent

active

060374659

ABSTRACT:
A universal process is disclosed for extracting and purifying nucleic acids from extremely small amounts of highly contaminated various biological and other starting materials. The invention has applications in forensic medicine, medical diagnosis, molecular biology, biochemistry, genetic technology and all related fields. The process is characterized in that nucleic acid-containing materials are lysed, the lysate is incubated with a non-porous, non-structured, highly disperse, homogeneous and chemically pure SiO.sub.2 substrate, the substrate is isolated with the bound nucleic acids and washed with a buffer solution, then the nucleic acids are dissolved from the substrate by a buffer with a lower salt concentration. Lysis of the material and nucleic acid immobilization are preferably carried out in a reaction vessel. The substrate particles have a size of 7-40 nm, preferably 40 nm, and a specific surface from 50-300 g/m.sup.2, preferably 50 g/m.sup.2.

REFERENCES:
patent: 5234809 (1993-08-01), Boom et al.

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