Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2005-09-01
2010-06-29
Riley, Jezia (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S004100, C536S023100, C536S025300, C536S023740, C532S001000, C564S012000, C977S792000, C435S006120
Reexamination Certificate
active
07745614
ABSTRACT:
A universal linker structure is provided, in which a functional group and activating leaving group are placed on a tether, allowing the placement of an electrophile at the end of any nucleic acid sequence. The electrophile on the tether can react with a second nucleic acid carrying a nucleophile when the two nucleic acids are hybridized near one another, resulting in release of the leaving group, and creation of a functional change. The linker can be designed to destabilize the ligation product without slowing the rate of reaction. This lowers product inhibition, and the target DNA or RNA can become a catalyst for isothermally generating multiple signals for detection. This enhanced signal is demonstrated in solution experiments and in solid supported assays. The universal linkers of the present invention are simple and inexpensive to prepare, and can be appended to any polynucleotide in automated steps on a standard DNA synthesizer.
REFERENCES:
patent: 4958013 (1990-09-01), Letsinger
patent: 5571903 (1996-11-01), Gryaznov
patent: 7109312 (2006-09-01), Cook et al.
patent: WO 2004/010101 (2004-01-01), None
Stratagene Catalog p. 39, 1988.
Brunner et al., DNA-Templated Metal Catalysis, J. Am. Chem. Soc., 2003, 125, 12410-12411.
Ficht et al., Single-Nucleotide-Specific PNA—Peptide Ligation on Synthetic and PCR DNA Templates, 2004, JACS, 126:9970-9981.
Gryaznov et al., Chemical Ligation of Oligonucleotides in the Presence and Absence of a Template, 1993, JACS 115:3808-3809.
Komatsu et al., In Vitro Selection of Hairpin Ribozymes Activated with Short Oligonucleotides, Biochemistry, 2002, 41, 9090-9098.
Kuhn et al., Brief Communication PNA Beacons for Duplex DNA, 2001, Antisense Nucleic Acid Drug Dev., 2001, 11:265-270.
Luther et al., Surface-promoted replication and exponential amplification of DNA analogues, Nature, 1998, 396, 245-248.
Ma et al., PNA-Based RNA-Triggered Drug-Releasing System, Bioconjugate Chem., 2003, 14, 679-683.
Ma et al., Nucleic Acid Triggered Catalytic Drug and Probe Release: A New Concept for the Design of Chemotherapeutic and Diagnostic Agents, Bioorg. Med. Chem., 2001, 9, 2501-2510.
Ma et al., Nucleic acid-triggered catalytic drug release, Proc. Natl. Acad. Sci., 2000, 97(21), 11159-11163.
Okamoto et al., Design of Base-discriminating Fluorescent Nucleoside and its Application to T/C SNP Typing, 2003, JACS, 125:9296-9297.
Paris et al., Probing DNA sequences in solution with a monomer-excimer fluorescence color change, 1998, N.A.R., 26:3789-3793.
Sando et al., Quencher as Leaving Group: Efficient Detection of DNA-Joining Reactions, 2002, J. Am. Chem. Soc., 124 (10): 2096-2097.
Sando et al., Amplified Nucleic Acid Sensing Using Programmed Self-Cleaving DNAzyme, J. Am. Chem. Soc., 2003, 125, 15720-15721.
Tyagi et al., Molecular Beacons: Probes that Fluoresce upon Hybridization, 1996, Nat. Biotech., 14:303-308.
Wang et al., A Novel Mode of Regulation of an RNA-cleaving DNAzyme by Effectors that Bind to Both Enzyme and Substrate, J. Mol. Biol., 2001, 310, 723-734.
Xu et al., A Novel 5′-Iodonucleoside Allows Efficient Nonenzymatic Ligation of Single-stranded and Duplex DNAs, 1997, Tetrahedron Lett., 38:5595-5598.
Xu et al., Rapid and Selective Selenium-Mediated Autoligation of DNA Strands, 2000, JACS, 122:9040-9041.
Xu et al., Nonenzymatic autoligation in direct three-color detection of RNA and DNA point mutations, 2001, Nat. Biotech., 19:148-152.
Zhan et al., Chemical Amplification through Template-Directed Synthesis, J. Am. Chem. Soc., 1997, 119, 12420-12421.
Abe Hiroshi
Kool Eric Todd
Bozicevic Field & Francis LLP
Riley Jezia
Sherwood Pamela J.
The Board of Trustees of the Leland Stanford Junior University
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