Universal G-protein coupled receptor reporter constructs

Details Universal G-protein coupled receptor reporter constructs Universal G-protein coupled receptor reporter constructs

2007-06-12

2007-06-12

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S320100, C435S325000

Reexamination Certificate

active

10759740

ABSTRACT:
The present invention provides universal G protein-coupled receptor (GPCR) reporter constructs. The constructs comprise a serum response element, a cAMP response element, and a multiple response element. These reporter constructs are able to detect activities of all GPCRs examined. Further provided by the present invention are host cells harboring a universal GPCR reporter construct of the invention, as well as assays for detecting modulators of GPCRs using the host cells.

REFERENCES:
Jiang et al. Generation of a bioactive neuropeptide in a cell-free system Anal. Biocehm 316 (2003) 34-40.
Friedman, et al. Processing of Prothyrotropin-Releasing Hormone (Pro-TRH) by Bovine Intermediate Lobe Secretory Vesicle Membrane PC1 and PC2 Enzymes. Ednocrinology, 1995. vol. 136, No. 10, pp. 4462-4472.
Nillni, et al. Posttranslational Processing of Progrowth Hormone-Releasing Hormone. Endocrinology, 1999. vol. 140, No. 12, p. 5817-5827.
MacDonald, et al. Posttranslational Processing of Alpha-, Beta-, and Gamma-Preprotachykinins. The Journal of Biological Chemistry, 1988. vol. 263, No. 29, Issue of Oct. 15, p. 15176-15183.
Schaner, et al. Processing of Prothyrotropin-releasing Hormone by the Family of Prohormone Convertases. The Journal of Biological Chemistry, 1997, vol. 272, No. 32, Issue of Aug. 8, p. 19958-19968.
Huynh, Hung. Post-transcriptional and post-translational regulation of insulin-like growth factor binding protein-3 and -4 by insulin-like growth factor-I in uterine myometrial cells. Growth Hormone & IGF Research 2000, vol. 10, p. 20-27.
Hook, et al. Production of Radiolabeled Neuropeptide Precursors by in vitro Transcription and Translation, Pept. Res. 9(4): pp. 183-187, 1996.
Fitzgerald et al., Analyt. Biochem. 275: 54-61, 1999.
White et al. Evaluation of the Effectiveness of DNA-Binding Drugs to Inhibit Transcription Using the c-fos Serum Response Element as a Target. Biochemistry, vol. 39, pp. 12262-12273, 2000.
Sho et al. Novel Roles of Specific Isoforms of Protein Kinase C in Activation of the c-fos Serum Response Element. Molecular and Cellular Biology, Feb. 1999, pp. 1313-1324.
Sealy et al. Regulation of the cfos Serum Response Element by C/EBP/β. Molecular and Cellular Biology, Mar. 1997, pp. 1744-1755.
Jiang et al. Generation of a bioactive neuropeptide in a cell-free system. Analytical Chemistry, 316, 2003, pp. 34-40.

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