Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-04-19
1988-11-15
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 435253, 435254, 435255, 435320, 536 27, 935 14, 935 27, 935 28, 935 29, 935 47, C12P 2100, C12N 1500, C12N 120, C07H 1512
Patent
active
047849495
ABSTRACT:
A novel universal dominant selectable marker cassette is disclosed. The marker comprises the coding sequences for aminoglycoside phosphotransferase I (APH-I) which has been modified and truncated so as to render its use in recombinant vectors more convenient. The modified, truncated sequence (mtAPH-I) gene is capable, upon expression, of conferring resistance to a number of antibiotics on the host. One of these antibiotics, G418, is toxic to eucaryotic as well as procaryotic hosts. Also disclosed are methods of constructing fusion proteins having N-terminal sequences corresponding to a desired peptide sequence, and C-terminal sequences comprising the amino acids encoded by mtAPH-I.
REFERENCES:
Southern et al 1982 "Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene . . . ", J Mol Appl Gen vl 327-341.
Oka et al 1981 "Nucleotide Sequence of the Kanamycin Resistance Transposon Tn 903", J Mol Biol v147 217-226.
Colbere-Garapin et al 1981 "A New Dominant Selective Marker for Higher Eukaryotic Cells", J Mol Biol v150 pp. 1-14.
Thompson et al 1983 "Nucleotide Sequence of a Streptomycete Aminoglycoside Phosphotransferase Gene . . . ", Proc Natl Acad Sci v80 5190-5194.
Jimenez et al 1980 "Expression of a Transposable Antibiotic Resistance Element in Saccharomyces", Nature v287 p. 869.
Fraley et al 1983 "Expression of Bacterial Genes in Plant Cells", Proc. Natl Acad Sci v80 4803-4807.
Webster et al 1983 "Direct Selection of Saccharomyces cerevisiae Resistant to the Antibiotic G418 . . . ", Gene v26 pp. 243-252.
Sharp, P. A., et al J. Mol. Biol. (1973) 75:235-255.
Jorgensen, R. A., et al Molec. Gen. Genet. 177, 65-72 (1979).
Gelfand David H.
Lawyer Frances C.
Stoffel Susanne
Cetus Corporation
Halluin Albert P.
Mays Thomas D.
Murashige Kate H.
Wiseman Thomas G.
LandOfFree
Universal dominant selectable marker cassette does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Universal dominant selectable marker cassette, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Universal dominant selectable marker cassette will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1103387