Universal biological indicator system

Details Universal biological indicator system Universal biological indicator system

1989-03-27

1989-12-05

Dority, Carroll B.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435294, 422101, C12M 124

Patent

active

048852538

ABSTRACT:
A cylindrical, transparent capsule (10) is less than half filled with a liquid culture medium (12). An open end of the capsule is closed by supporting annulus (24) and a vent tube (28) having a vent aperture (30) at a free end thereof. The vent aperture is disposed at a volumetric center of the capsule such that an upper surface of the liquid culture medium is disposed below the vent aperture in any orientation of the capsule (FIG. 2). A heat labile membrane (32) which becomes porous in the presence of steam seals the vent passage but provided a pressure escape from the capsule at steam sterilizing temperatures. A disk inoculated with microorganisms is supported by the annular support. A cap (40) is slidably movable between a first position (FIG. 1) in which apertures (42) provide a liquid flow path between the inoculated disk and the exterior and a second position (FIG. 3) in which a severing ring (46) severs the support annulus bringing the inoculated disk and the culture medium into contact (FIG. 4).

REFERENCES:
patent: 3661717 (1972-05-01), Nelson
patent: 4345028 (1982-08-01), Nelson et al.
patent: 4461837 (1984-07-01), Karle et al.
patent: 4534939 (1985-08-01), Smith et al.
patent: 4579823 (1986-01-01), Ryder
patent: 4596773 (1986-06-01), Wheeler
patent: 4743537 (1988-05-01), McCormick
patent: 4810651 (1989-03-01), Schwartz

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