Universal binding film

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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422 681, 422 8205, 427327, 427328, 427402, 428615, 428621, 428624, 435 4, 435 75, 436518, 436524, 436525, 436527, G01N 3353, G01N 33543, B05D 310, B32B 1500

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active

057631913

ABSTRACT:
The invention concerns a binding matrix containing a carrier material and a solid phase reactant which is adsorbed to this via anchor groups that is capable of binding to at least one free reaction partner wherein the solid phase reactant forms a dilute and essentially laterally homogeneous binding layer on the surface of the carrier material. In addition a method for the determination of an analyte in a sample solution is claimed in which a solid phase reactant is used which is a component of a binding matrix according to the present invention. In this process the specific binding reaction is preferably determined by optical reflection techniques.

REFERENCES:
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Richard C. Ebersole et al. "Spontaneously Formed Functionally Active Avidin Monolayers on Metal Surfaces: A Strategy for Immobilizing Biological Reagents and Design of Piezoelectric Biosensors" J. Am. Chem. Soc. (1990) 112:3239-3241.
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