Unitary sandwhich enzyme immunoassay cassette device and method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 5, 435 6, 435 71, 435 79, 435 794, 435975, 422 8201, 422 8202, 436518, 436525, 436528, 436807, G01N 3353

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active

058306803

ABSTRACT:
A novel enzyme sandwich immunoassay cassette comprising a microporous membrane support having coated thereon:

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Duan, C. et al., "Separation-Free Sandwich Enzyme Immunoassays Using Microporous Gold Electrodes and Self-Assembled Monolayer/Immobilized Capture Antibodies," Analytical Chemistry, 66(9):1369-1377 (1994).
Valkirs et al., "Qualitative Two-site IEMA for Serum hCG Using Immunoconcentration Technology", Clin. Chem. 31, 960 (1985). Abstract No. 300.
Valkirs et al., "Immunoconcentration--a New Format for Solid-Phase Immunoassays", Clin. Chem. 31, 1427-31 (1985).
Gibbons et al., "Homogenous Enzyme Immunoassay for Proteins Employing .beta.-Galactosidase", Anal. Biochem. 102, 167-170 (1980).
Armenta et al., "Improved Sensitivity in Homogenous Enzyme Immunoassays Using a Fluorogenic Macromolecular Substrate: An Assay for Serum Ferritin", Anal. Biochem. 146, 211-219 (1985).
Chen et al., "Ultrasound-Accelerated Immunoassay, as Exemplified by Enzyme Immunoassay for Choriogonado-tropin", Clin. Chem. 30, 1446-41 (1984).
Schray et al., "Separation-Free Dual Solid Phase Enzyme Immunoassay for Macromolecules", Anal. Chem., 60 358-56 (1988).

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