Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1992-04-28
1994-10-25
Nucker, Christine M.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530834, 530806, 530828, 530412, 436174, 436177, C07K 1500
Patent
active
053590317
ABSTRACT:
The present invention involves a method for detecting bladder cancer in a subject. The method preferably comprises first collecting a urine sample from the subject. The presence of a proteinaceous substance having a molecular weight of about 180 kDa according to its relative electrophoretic migration rate through detergent-containing polyacrylamide gel is then measured. This substance reversibly binds concanavalin A and is complexed with gamma globulin while in the urine. The gamma globulin complex binds to Staphlococcal protein A. Said proteinaceous substance, when present in detectable amount, is an indicator of bladder cancer.
REFERENCES:
Stanker et al: One-Step Purification . . . Chromatography J. Immunol Meth 76(1985) 157-169.
Burkovsky et al: Simple & Rapid . . . Scales Hybridoma V6 #2 1987 pp. 219-228.
DeFazio: Tumor-Associated Antigens in the Urine of Patients w/ Bladder Cancer. Cancer Research V42 1982 pp. 2913.sub.]2917.
Johansson, B. et al., "Proteinuria in patients with urinary tract tumors." Scand. J. Urol. Nephrol., 5:229-233, 1971, published in Sweden.
Hakala, T. R. et al., "Humoral cytotoxicity in human transitional cell carcinoma." J. Urol., 111:382-385, 1974, published in USA.
Troye, M. et al., "The use of Fab fragments of anti-human immunoglobulin as analytic tools for establishing the involvement of immunoglobulin in the spontaneous cytotoxicity to cultured tumor cells by lymphocytes from patients with bladder carcinoma and from health donors." J. Immunol., 119:1061-1067, 1977, published in USA.
Gozzo, J. et al., "Use of heterogeneous and monospecific antisera for the diagnosis of bladder cancer." J. Urol., 118:748-751, 1977, published in USA.
O'Brien, P. et al., "Qualitative analysis of proteinuria associated with bladder cancer." Invest. Urol., 17:28-32, 1979, published in USA.
Wahren, B. et al., "Tumor markers in gastrointestinal and urothelial cancers." In Tumor Markers: Impact and Prospects., E. Boelsma and P. Rumke, eds. pp. 267-279, 1979, published in the Netherlands.
Betkerur, V. et al., "Screening tests for detection of bladder cancer." Urology, 16:16-19, 1980, published in USA.
Betkerur, V. et al., "Urinary immunoglobulin A in the diagnosis of bladder cancer." J. Surg. Oncol., 16:215-217, 1981, published in USA.
Huland, H. et al., "The value of urinary cytology, serum and urinary carcinoembryogenic antigen, rheumatoid factors, an urinary immunoglobulin concentration as tumor markers or prognostic factors in predicting progression of superficial bladder cancer." Eur. Urol., 9:346-349, 1983, published in Switzerland.
Langone, J. J. et al., "Applications of immobilized protein A in immunochemical techniques." J. Immunol. Methods, 55:277-296, 1982, published in the Netherlands.
Laemmli, U. K. et al., "Cleavage of structural proteins during the assembly of the head of bacteriophage T4." Nature, 227:680, 1970, published in U.K.
Lowry, O. H. et al., "Protein measurement with the folin phenol reagent." J. Biol. Chem., 193:265, 1951, published in USA.
Towbin, H. et al., "Immunoblotting and dot immunobinding: current status and outlook". J. Immunol. Methods, 72:313, 1984, published in the Netherlands.
Ey, P. L. et al., "Isolation of pure IgG.sub.1, IgG.sub.2a and IgG.sub.2b immunoglobulins from mouse serum using protein A-SEPHAROSE." Immunochemistry, 15:429-436, 1978, published in Great Britain.
Kobayashi, K. et al., "Circulating immune complexes in patients with bladder cancer and other malignancies of the urogenital tract." Urol. Int., 39:232, 1984, published in Switzerland.
Hemmingsen, L. et al., "Urinary protein profiles in patients with urothelial bladder tumors." Br. J. Urol., 53:324-329, 1981, published in Great Britain.
Mayes, E. L. V. et al., "Biosynthesis of the epidermal growth factor receptor in A431 cells." EMBO, 3:531-537, 1984, published in England.
Gozzo, J. J. et al., "Detection of tumor-associated antigens in urine from patients with bladder cancer." J. Urol., 124:804, 1980, published in USA.
Rote, N. S. et al., "Tumor associated antigens detected by autologous sera in urine of patients with solid neoplasms." J. Surg. Res., 29:18-22, 1980, published in USA.
Rife, C. C. et al., "Urine cytology of transitional cell neoplasms." Urol. Clin. North Am., 6:599-612, 1979, published in USA.
Matthews, D. E. et al., "Using and Understanding Medical Statistics." Basel, New York: Karger, pp. 20-26, 1985, published in Switzerland.
Johansson, B. et al., "Proteinuria in patients with uroepithelial tumors with special regard to tumor size, clinical staging and grade of malignancy." Scand. J. Urol. Nephrol., 9:52, 1974, published in Sweden.
Chao, H. E. et al., "Neonatal Imprinting and Hepatic Cytochrome," p. 450, Molecular Pharmacology 21:744-752, 1982, published in USA.
Kohler, G., and Milstein, C., "Nature" (Lond.) 256-495, 1975, published in U.K.
Chan, J. C., Keek, M. E., and Li, W. J., "Biochem, Biophys. Res. Comm.", 134:1223, 1986, published in USA.
Hsu, S. M. et al., "A Comparative Study of the Peroxidase-antiperoxidase Method and an Avidin-Biotin Complex Method for Studying Polypeptide Hormones with Radioimmunoassay Antibiodies," Am. J. Clin. Pathol., 75:734-738, 1981, published in USA.
Higashi, G. et al., "Preliminary Identification of a Tumor-Associated Glycoprotein and Bilharzial Bladder Cancer Urine." Int. J. Cancer, 32:445-448, 1983, published in USA.
Braesch-Anderson, S. et al., "Isolation and Characterization of Two Bladder Carcinoma-Associated Antigens." J. Immunol. Method., 94:145-151, 1986, published in the Netherlands.
Koho, H. et al., "Monoclonal Antibodies to Antigens Associated with transitional Cell Carcinoma of the Human Urinary Bladder." Cancer Immunol. Immunother., 17:165-172, 1984, published in Germany.
Paulie, S. et al., "Monoclonal Antibodies to Antigens Associated with Transitional Cell Carcinoma of the Human Urinary Bladder." Cancer Immunol. Immunother., 17:173-179, 1984, published in Germany.
Babaian, R. J. et al., "Immune Complexes in Urine and Serum of Patients with Bladder Cancer." J. Urol., 131:463-466, 1984, published in USA.
Hellstrom, I. et al., "Monoclonal Antibodies to Cell Surface Antigens Shared by Chemically Induced Mouse Bladder Carcinomas." Chem. Abstr., 102:462, 1985, published in USA.
Lee, V. K. et al., "Monoclonal Antiidiotypic Antibodies Related to a Murine Oncofetal Bladder Tumor Antigen Induce Specific Cell-Mediated Tumor Immunity." Proc. Natl. Acad. Sci., 82:6286, 1985, published in USA.
Zhau, H. et al., "Detection of Immunoglobulin Reactivity in Urine Specimens of Bladder Cancer Patients," J. Urol., 139:241a, 1988, published in USA.
Guirguis, R. et al., "Detection of Autocrine Motility Factor in Urine as a Marker of Bladder Cancer," J. of the Natl. Canc. Inst., 80(15):1203-1211, 1988, published in USA.
Babaian Richard J.
Zhau Haiyen E.
Board of Regents , The University of Texas System
Nucker Christine M.
Stucker Jeffrey
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