Unique nucleotide and amino acid sequence and uses thereof

Details Unique nucleotide and amino acid sequence and uses thereof Unique nucleotide and amino acid sequence and uses thereof

1997-01-30

2000-01-25

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 914, 4353201, 435348, 435365, 536 231, 536 2372, 536 241, C07H 2100, C12N 510, C12N 1533, C12N 1563

Patent

active

060177349

ABSTRACT:
Provided are hydrophobic targeting sequences, which may serve to target heterologous proteins to a variety of cellular membranes. In particular, the structural components of the nuclear envelope, or those components which become nucleus-associated, may be targeted with the sequences provided. Also provided are methods of targeting heterologous proteins to particular membranes, and the use of these targeted proteins in therapeutic, diagnostic and insecticidal applications.

REFERENCES:
Harvey et al. (1983) Biochemical and biological variation of Cydia pomenella (Coding Moth) granulosis virus. (Virology 124:21-34.
Kuzio et al. (1984) Regions of repeated DNA in the genome of Choristoneura fumiferana nuclear polyhedrosis virus. Virology 139:185-188.
Ma et al. (Jan. 1993) Cloning and sequence analysis of a p40 structural protein gene of Helicoverpa zea nuclear polyhedrosis virus. Virology 192:224-233.
Kondo et al. (Jul. 1991) Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus. J. Virol. 65:3625-3632.
Leisy et al. (Nov. 1984) Conservation of genome organization in two multicapsid nuclear polyhedrosis viruses. J. Virol. 52:699-702.
Camacho et al. (1991) Protein p22 of African Swine Fever Virus: an early structural protein that is incorporated into the membrane of infected cells. Virology 181:251-257.
Friedmann (Jun. 1997) Overcoming the obstacles to gene therapy. Scientific Amer. Jun.:96-101.
Adams, J.R., and McClintock, J. T.; Baculoviridae. Nuclear Polyhedrosis Virus, In "Atlas of Invertebrate Viruses" (J.R. Adams and J. R. Bonami, Eds.). CRC Press, Boca Raton, FL. (1991) 87-204.
Ahmad, S., Bassiri, M., Banerjee, AK., Yilma, T. (1993). Immunological Characterization of the VSV Nucleocapsid (N) Protein Expressed by Recombinant Baculovirus in Spodoptera exigua Larva: Use in Differential Diagnosis Between Vaccinated and Infected Animals. Virology.192:207-216.
Arvinte T., Schulz B., Cudd A. Nicolau C. (1989). Low pH association of proteins with the membranes of intact red blood cells. I. Exogenous glycophorin and the CD4 molecule. Biochim. Biophys. Acta 981:51-60.
Ayres, M. D., Howard, S. C., Kuzio, J., Lopez-Ferber, M., and Possee, R. D. (1994). The Complete DNA Sequence of Autographa californica Nuclear Polyhedrosis Virus. Virology. 202:586-605.
Belyaev, A. S., and Roy, P. (1993). Development of baculovirus triple and quadruple expression vectors: co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells. Nucleic Acids Res. 21:1219-1223.
Blissard, G. W., and Rohrmann, G. F. (1989). Location, Sequence, Transcriptional Mapping, and Temporal Expression of the gp64 Envelope Glycoprotein Gene of the Orgyia pseudotsugata Multicapsid Nuclear Polyhedrosis Virus. Virology.170:537-555.
Blissard, G.W., and Rohrmann, G. F., (1990). Baculovirus Diversity and Molecular Biology. Annu. Rev. Entomol. 35:127-155.
Bozzola, J. J., and Russell, L. D. (1992). Electron Microscopy. Jones and Bartlett Publishers, Boston, MA.
Bradford, M. M. (1976). A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem. 72:248-254.
Braunagel, S. C., et al. (1992). Sequence, Genomic Organization of the EcoR1-A Fragment Autographa californica Nuclear Polyhedrosis Virus, and Identification of a Viral Encoded Protein Resembling the Outer Capsid Protein VPS of Rotavirus, Virology. 191:1003-1008.
Braunagel, S. C., et al. (1996). Identification and Anaylsis of an Autographa californica Nuclear Polyhedrosis Virus Structural Protein of the Occlusion-Derived Virus Envelope ODV-E35, and ODV-EC27, Virology. 222:(in press).
Braunagel, S. C., et al. (1996). Transcription, Translation and Cellular Localization of Three Autographa californica Nuclear Polyhedrosis Virus Structural Proteins: ODV-E18, ODV-E35, and ODV-EC27, Virology. 222:100-114.
Braunagel, S. C., et al. (1994). Autographa californica Nuclear Polyhedrosis Virus, PDV, and ECV Viral Envelopes and Nucleocapsids: Structural Proteins, Antigens, Lipid and Fatty Acid Profiles. Virology. 202:315-328.
Carrington, J. C., and Freed, D. D. (1990). Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region. J. Virol.64:1590-1597.
Carson, D. D., Guarino, L. A., and Summers, M. D. (1988). Functional Mapping of an AcMNPV Immediate Early Gene Which Augments Expression of the IE-1 trans-Activated 39K Gene. Virology. 162:444-451.
Carson, D.D., Summers, M. D., and Guarino, L. A. (1991). Transient Expression of the Autographa californica Nuclear Polyhedrosis Virus Immediate Early Gene, IE-N, Is Regulated by Three Viral Elements. J. Virol. 65:945-951.
Charlton, C. A., and Volkman, L. E. (1993). Penetration of Autographa californica Nuclear Polyhedrosis Virus Nucleocapsids into IPLB Sf21 Cells Induces Actin Cable Formation. Virology. 197:245-254.
Choi, J., and Guarino, L. A. (1995). A Temperature-Sensitive IE1 Transactivation and DNA Binding Activities. Virology. 209:90-98.
Choi, J., and Guarino, L. A. (1995). Expression of the IE1 Transactivator of Autographa californica Nuclear Polyhedrosis Virus During Viral Infection. Virology. 209:99-107.
Choi, J., and Guarino, L. A. (1995). The Baculoviruses Transactivator IE1 Binds to Viral Enhancer Elements in the Absence of Insect Cell Factors. J. Virol. 69:4548-4551.
Cory, J. S., and Bishop, D. H. L. (1995). Use of Baculoviruses as Biological Insecticides, In "Methods in Molecular Biology". vol.39, pp. 277-294, (C. D. Richardson, ed.). Humana Press, Inc., Totowa, N.J.
Devereux, J., Haeberli, P., and Smithies, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucl. Acids Res. 12:389-395.
Dewey, R. E., Levings, C. S., III, and Timothy, D. H. (1986). Novel Recombinations in the Maize Mitochondrial Genome Produce a Unique Transcriptional Unit in the Texas Male-Sterile Cytoplasm. Cell. 44:439-449.
Funk, C. J., and Consigli, R. A. (1993). Temporal expression and immunogold localization of Plodia interpunctella granulosis virus structural proteins. Virus Res. 28:57-66.
Furukawa, K., Pante, N., Aebi, U., and Gerace, L. (1995). Cloning of a cDNA for lamina associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope. EMBO J. 14:1626-1636.
Gilbert, R., Ghosh, K., Rasile, L., and Ghosh, H. P. (1994). Membrane Anchoring Domain of Herpes Simplex Virus Glycoprotein gB Is Sufficient for Nuclear Envelope Localization. J. Virol. 68:2272-2285.
Gluzman, Y. (1981). SV40-Transformed Simian Cells Support the Replication of Early SV40 Mutants. Cell. 23:175-182.
Groner, A. (1986). Specificity and safety of baculoviruses. In "The Biology of Baculoviruses." pp. 177-202, (R. R. Granados and B. A. Federici, eds.). CRC Press, Boca Raton, FL.
Guarino, L. A., Smith, G., and Dong, W. (1995). Ubiquitin is Attached to Membranes of Baculovirus Particles by a Novel Type of Phospholipid Anchor. Cell. 80:301-309.
Harel, A., Zlotkin, E., Nainudel-Epszteyn, S., Feinstein, N., Fisher, P. A., and Gruenbaum, Y. (1989). Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in Drosophila melanogaster embryos. J. Cell Sci. 94:463-470.
Harlow, E., and Lane D. (1988). Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 92-115.
Hershberger, P. A., LaCount, D. J., and Friesen, P. D. (1994). The Apoptotic Suppressor P35 Is Required Early During Baculovirus Replication And Is Targeted to the Cytosol of Infected Cells. J. Virol. 68:3467-3477.
Hill, J. E., and Faulkner, P. (1994). Identification of the gp67 gene of a baculovirus pathogenic to the spruce budworm, Choristoneura fumiferana multinucleocapsid nuclear polyhedrosis virus. J. Gen. Virol. 75:1811-1813.
Hong, T. (1995). Studies on a PDV Envelope Protein of Autographa californica Nuclear Polyhedrosis Virus. Ph.D dissertation, Department of Biology, Texas A&M University, College Station, Texas.
Hong, T., Brauna

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