Unglycosylated recombinant human lymphotoxin polypeptides and co

Details Unglycosylated recombinant human lymphotoxin polypeptides and co Unglycosylated recombinant human lymphotoxin polypeptides and co

1992-02-14

1997-11-04

Fitzgerald, David L.

Drug, bio-affecting and body treating compositions

Lymphokine

530 351, 435 695, 930143, A61K 3819, C07K 1452, C07K 14525

Patent

active

056836880

ABSTRACT:
Biologically active lymphotoxin polypeptides are synthesized in recombinant cell culture. Novel nucleic acid and vectors incorporating same are provided. The compositions and processes herein enable the economical preparation of compositions containing uniform lymphotoxin polypeptides and variant lymphotoxins having amino acid sequences that differ from those found in nature. The lymphotoxins are purified to a specific activity of 2-10.times.10.sup.7 units/mg of protein by purification using a novel immobilized, lymphotoxin-neutralizing monoclonal antibody.

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