Uncoupling of DNA insert propagation and expression of...

Details Uncoupling of DNA insert propagation and expression of... Uncoupling of DNA insert propagation and expression of...

2006-09-26

2006-09-26

Priebe, Scott D. (Department: 1633)

Chemistry: molecular biology and microbiology

Vector, per se

C435S005000, C435S006120, C435S472000, C435S475000, C435S476000, C536S023400

Reexamination Certificate

active

07112435

ABSTRACT:
The present invention provides an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of said polypeptides. The invention provides phage constructs and methods for their use to permit phage coat protein expression, and thus phage propagation, in the absence of display of heterologous polypeptides, which may be expressed as a fusion with said coat protein in a regulated manner.

REFERENCES:
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5403484 (1995-04-01), Ladner et al.
patent: 5498538 (1996-03-01), Kay et al.
patent: 5571698 (1996-11-01), Ladner et al.
patent: 5693489 (1997-12-01), Studier et al.
patent: 5766905 (1998-06-01), Studier et al.
patent: 5770356 (1998-06-01), Light et al.
patent: 5837242 (1998-11-01), Holliger et al.
patent: 5885793 (1999-03-01), Griffiths et al.
patent: 6172197 (2001-01-01), McCafferty et al.
patent: 2002/0197606 (2002-12-01), Craig
patent: 2002/0198162 (2002-12-01), Punnonen et al.
patent: 2003/0108885 (2003-06-01), Schultz et al.
patent: 2003/0186221 (2003-10-01), Lockhart et al.
patent: WO 01/18234 (2001-03-01), None
Hoogenboom et al. “Multi-Subunit Proteins on the Surface of Filamentous Phage: Methodologies for Displaying Antibody (Fab) Heavy and Light Chains” Nucl. Acids. Res. 19(15):4133-4137 (1991).
Huang et al. “Use of the Arabinose PbadPromoter for Tightly Regulated Display of Proteins on Bacteriophage” Gene 251:187-197 (2000).
Lucic et al. “Secretion inEscherichia coliand Phage-Display of Recombinant Insulin-Like Growth Factor Binding Protein-2” J. Biotech 61:95-108 (1998).
Mikawa et al. “Surface Display of Proteins on Bacteriophage 2 Heads” J. Mol. Biol. 262:21-30 (1996).
Ward et al. “Retrieval of Human Antibodies from Phage-Display Libraries Using Enzymatic Cleavage” J. Imm. Meth. 189(1):73-82 (1996).
International Search Report, mailed on Dec. 19, 2003, for PCT patent application No. PCT/US03/24221, filed on Aug. 1, 2003, 5 pages.
Condron et al., An analysis of sequences stimulating frame shifting in the decoding of gene 10 of bacteriophage T7 Nucleic acid Res. 19 (20) 5607-12 (1991).
Condron et al., Frame shifting in gene 10 of bacteriophage T7 J. Bacteriol. 173 (21) 6998-7003 (1991).
Sipley et al., Bacteriophage T7 morphogenesis and gene 10 frame shifting inEscherichia colishowing different degrees of ribosomal fidelity Gen. Genet. 230 (3) 376-84 (1991).
Pope et al., In vitro selection of a high affinity antibody to oestradiol using a phage display human antibody library Immunotech. (1996) 209-217.
Rosenberg, A et al., T7 Select Phage display system: A powerful new protein display system based on bacteriophage T7 inNovations 6 (Dec. 1996).
Vaughan et al., Human antibodies with sub-nanomolar affinities isolated from a large non immunized phage display library Nat. Biotech. (1996) 14 309-314.
Hoogenboom, H. Designing and optimizing library selection strategies for generating high affinity antibodies. TIBTECH (Feb. 1997) 15 62-70.
Nilsson, J et al., Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Prot. Exp. & Pur. (1997) 11 1-16.
Hoogenboom, H et al., Antibody phage display technology and its applications Immunotech. (1998) 4 1-20.
De Haard et al., A large non-immunized human Fab fragment phage library that permits .. J. Biol. Chem. (1999) 274 (26) 18218-28230.
Wycuff et al. Generation of AraC-araBAD promoter-regulated T7 expression system Analytical Biochemistry (2000) 277 67-73.
Lowman, H.B. “Bacteriophage Display and Discovery of Peptide Leads for Drug Development” (1997) vol. 26: 401-424.

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