Unactivated ungulate oocytes to produce a cloned ungulate by...

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of a nucleus into an embryo – egg cell – or...

Reexamination Certificate

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C800S008000, C800S015000, C800S016000, C800S017000

Reexamination Certificate

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10914161

ABSTRACT:
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0or G1phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.

REFERENCES:
patent: 5057420 (1991-10-01), Massey
patent: 5496720 (1996-03-01), Susko-Parrish et al.
patent: 5523226 (1996-06-01), Wheeler
patent: 5945577 (1999-08-01), Stice et al.
patent: 6147276 (2000-11-01), Campbell et al.
patent: 6215041 (2001-04-01), Stice et al.
patent: 6235969 (2001-05-01), Stice et al.
patent: 6235970 (2001-05-01), Stice et al.
patent: 6252133 (2001-06-01), Campbell et al.
patent: 6525243 (2003-02-01), Stockman Campbell et al.
patent: 6548741 (2003-04-01), DeSousa et al.
patent: 6603059 (2003-08-01), Strelchenko et al.
patent: 2092258 (1993-03-01), None
patent: 2059199 (1993-07-01), None
patent: GB 2 265 909 (1993-10-01), None
patent: WO A 94 06422 (1994-03-01), None
patent: WO 94 18344 (1994-08-01), None
patent: WO A 95 03795 (1995-02-01), None
patent: WO 95 17500 (1995-06-01), None
patent: WO 96 07732 (1995-09-01), None
Mitalipov et al. Rhesus Monkey Embryos Produced by Nuclear Transfer from Embryonic Blastomeres or Somatic Cells. Biol. Reproduct. 2002, vol. 66, pp. 1367-1373.
Pennisi and Vogel. Cloning: A Hard Act to Follow. Science. Jun. 9, 2000, vol. 288, pp. 1722-1727.
Fitchev et al. Nuclear Transfer in the Rat: Potential Access to the Germline. Transplantation Proceed. 1999, vol. 31, pp. 1525-1530.
Chense et al. Cloned Rabbits Produced by Nuclear Transfer from Adult Somatic Cells. Nature Biotechnology. Apr. 2002, vol. 20, pp. 366-369.
Wakayama et al. Full-Term Development of Mice from Enucleated Oocytes Injected with Cumulus Cell Nuclei. Nature. Jul. 23, 1998, vol. 394, pp. 369-374.
Zhou et al. Generation of Fertile Cloned Rats by Regulating Oocyte Activation. Science. Nov. 14, 2003, vol. 302, pp. 1179.
Simerly et al. Molecular Correlates of Primate Nuclear Transfer Failures. Science. Apr. 11, 2003, vol. 300, pp. 297.
Campbell, K.H.S. Nuclear Transfer in Farm Animal Species. Seminars in Cell & Develop. Biol. 1999, vol. 19, pp. 245-252.
Araki, K., et al., “Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase,”Proc. Natl. Acad. Sci., USA, 92:160-164, Jan. 1995.
Campbell et al., “The science of providing milk for man,”Science Library(May 28, 1995).
Sims et al., “Production of calves by transfer of nuclei from cultured inner cell mass cell,”Developmental Biology, 90:6143-6147 (Jun. 1993).
Czolowska et al., “Behavior of thymocyte nuclei in non-activated and activated mouse oocytes,”Journal of Cell Science, vol. 69, see p. 27, line 1—p. 33, line 3; table 1 (1984).
Collas et al., “Factors affecting the efficiency of nuclear transplantation in the rabbit embryo,”Biology of Reproduction, 43(5):877-884 (Nov. 1990).
Campbell et al., “Nuclear-cytoplasmic interactions during the first cycle of nuclear transfer reconstructed bovine embryos: implications from desoxyribonucleic acid replication and development,”Biology of Reproduction, 49(1):933-942 (Nov. 1993).
Otaegui et al., “Transfer of nuclei from 8-cell stage mouse embryos following use of nocodazole to control the cell cycle,”Molecular Reproduction and Development, 39(2):147-152 (Oct. 1, 1994).
Willadsen. “Nuclear transplantation in sheep embryos,”Nature, 320(6), London, GB (Mar. 1986).
Greaham, “The fusion of cells with one-and two-cell mouse embryo,” The Wistar Institute Symposium Monograph, vol. 9 (Jan. 1, 1969).
Campbell et al., “Live lambs by nuclear transfer from an established cell line,”Theirogenology, 45(1):287, ISSN: 0083-691X (1996).
Campbell et al., “Sheep cloned by nuclear transfer from a cultured cell line,”Nature, 380:64 (Mar. 7, 1996).
Hoppe et al.,Proc. Natl'l Acad. Sci. USA, 79:1912-1916 (1982).
Modlinski et al.,Development, 108:337-348 (1990).
Illmensee et al.,Cell, 23:9-18 (1981).
Prather et al.,Biology of Reproduction, 41:414-418 (1989).
Hope et al.,Proc. Natl'l Acad. Sci. USA, 74:5657-5661 (1977).
Bromhall, J.D., “Nuclear transplantation in the rabbit egg,”Nature, 258(5537):719-722 (Dec. 25, 1975).
Prather,J., Exp. Zool, 225:355-358 (1990).
Richie et al.,J. Reprod. Fertil. Abstract Series 5(60), Abstract No. 175 (1995).
Robertson, E.J., “Teratocarcinomas&embryonic stem cells: a practical approach,” 71-112, IRL Press Oxford (1987).
Robl, Symposium on Cloning Mammals by Nuclear Transplantation (Seidel ed.)Colorado State University, pp. 24-27 (1992).
Saito et al.,Develop. Biol., 201:134-141 (1992).
Seamark,Reprod. Fertil.&Devel. 6(5):653, Abstract No. 95:4100 (1994).
Smith & Wilmut,Biol. Reprod., 40:1027-1035 (1989).
Solter,Nature, 380:24-25 (1996).
Spemann, Embryonic Development & Induction, Hofner Publishing Co., New York, pp. 210-211 (1938).
Stanton,Brain Physiology, 2:71-73 (1992).
Thompson,Biology of Reproduction, 53:1385-1391 (1995).
Tsunoda,J. Reprod. Fertil., 82:173 (1988).
Tsunoda,J. Reprod.&Fertil., 98:537-540 (1993).
Westhusin,Biol. Reprod.(Suppl.), 42:176, Abstract No. 407, (1990).
Wheeler,Reprod. Fertil. Dev., 6:563-568 (1994).
Whitfield,Control of Animal Cell Proliferation, 1:331-365 (1985).
Whittingham & Wales,Aust. J. Biol. Sci., 22:1065-1068 (1969).
Willadesen, In Mammalian Egg Transfer (Adams, E.E., ed.) 185, CRC Press, Boca Raton, Florida (1982).
Bradley,Biotechnology, 10:534-539 (1992).
Briggs & King,Proc. Nat'l. Acad. Sci. USA, 38:455-461 (1952).
Campbell & Wilmut,Vth World Congress on Genetics as Applied to Livestock, 20:180-187 (1994).
Campbell,Biol. Reprod., 50:1385-1393 (1994).
Campbell,Therio., 43:181 (1995).
Campbell,J. Reprod. Fertil. Abstract Series 5(31), Abstract No. 86 (1995).
Collas & Robl,Biol. Reprod., 45:455-465 (1991).
Cutbertson & Cobbold,Nature, 316:541-542 (1985).
Finch,Biochem Soc. Trans., 24:369S (1996).
Freeman & Beitz, In Symposium on Cloning Mammals by Nuclear Transplantation (Seidel, GE Jr. ed.), Colorado State University, Colorado, pp. 17-20 (1992).
Gardner,Biology of Reproduction, 50:390-400 (1960).
Gurdon,QJ Microsc. Soc., 101:299-311 (1960).
Kanka, Mol.Reprod. Dav., 29:110-116 (1991).
Leno & Munshi,J. Cell Biol., 127(1):5-14 (1994).
Prather & First,J. Reprod. Fertil. Suppl., 41:125 (1980).
Fulka et al.,BioEssays, 20:847-851 (1998).
Wilmut et al.,Nature, 385:810-813 (1997).
Prather,Biol. Reprod., 37:859-866 (1987).
Sims et al.,Proced. Natl'l. Acad. Sci., 90:6143-6147 (1993).

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