Ultraviolet imaging method and apparatus

Chemistry: analytical and immunological testing – Optical result

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355 85, 355113, 422 68, 204403, G01N 2726, G01N 3348

Patent

active

045392978

ABSTRACT:
A method for providing an image of zones of ultraviolet absorbing material in a medium substantially transparent to ultraviolet light, such as nucleic acid in a polyacrylamide gel, comprises the steps of placing in juxtaposition to the gel standard photographic film and directing ultraviolet light to the gel such that the photographic film is in the path of light exiting the gel. The zones in the gel absorb one or more discrete wavelengths of ultraviolet light, and the film is exposed by incident ultraviolet light such that a photographic image of the nucleic acid zones is created on the photographic film. Apparatus for providing ultraviolet images of ultraviolet light absorbing material in a medium relatively transparent to ultraviolet light comprises an enclosure in which a medium with zones of ultraviolet light absorbing material is placed. The closure contains an aperture for reception of light from an ultraviolet light source and is adapted to accommodate a strip of photographic film juxtaposed to the gel. The closure may include a slot giving access to the medium to enable the zones to be cut out of the medium upon inspection of the image thereof on the film.

REFERENCES:
patent: 3797934 (1974-03-01), Miller
patent: 3975636 (1976-08-01), Klein
patent: 4129380 (1978-12-01), Fohl
S. J. Luner, Anal. Biochem., 23, 357-358, (1968).

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