Ultrasensitive immunoassays

Details Ultrasensitive immunoassays Ultrasensitive immunoassays

2005-04-12

2005-04-12

Smith, Lynette R. F. (Department: 1645)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S091100, C435S091200, C435S007920, C435S007940, C435S007930, C435S975000, C435S810000, C436S518000, C436S501000, C514S04400A, C530S350000, C530S387100, C530S388100

Reexamination Certificate

active

06878515

ABSTRACT:
The present invention relates to an immunological test kit and immunoassay using a first immobilized antibody having affinity for a specific antigen. The invention is characterized by a second and third antibody being specific for different determinants of the antigen and modified with cross-linkable oligonucleotides. For detection, the oligonucleotides are amplified, whereby only such oligonucleotides will be amplified which have been cross-linked to each other. In this way unspecific background is avoided and detection is possible down to single molecules.

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