Ultrasensitive enzymatic radioimmunoassay method

Details Ultrasensitive enzymatic radioimmunoassay method Ultrasensitive enzymatic radioimmunoassay method

1979-05-31

1981-09-15

Kyle, Deborah L.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 424 12, 435 7, G01N 3348, G01T 100, G01N 3354

Patent

active

042897483

ABSTRACT:
A method of quantitatively measuring antigen or antibody concentration by selectively coupling an antigen to a solid substrate, coupling an enzyme-conjugated antibody to the antigen and reacting a radioindicator with the enzyme-conjugate producing a radiolabelled product. The product is scintillation counted to yield the measurement. Direct or indirect coupling of the antigen to the substrate can be used. Direct or indirect coupling of the enzyme-conjugate can be used. The preferred radioindicator is adenosine-5'-monophosphate used with alkaline phosphatase. Others are presented.

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