Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1993-01-26
1994-08-16
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
530412, 530415, 530416, 530417, C07K 302, C07K 320, C07K 322, C07K 1506
Patent
active
053388348
ABSTRACT:
Essentially pure human interleukin-6 having a purity of at least 98 % as determined by single peak migration using capillary electrophoresis is obtained by a 3-step purification method which includes a cation-exchange chromatography step, a hydrophobic chromatography step and a reverse-phase chromatography step in the presence of a charge modifier.
REFERENCES:
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Lee et al., "A Multifunctional Regulator Of Growth And Differentiation", Annals New York Academy Of Sciences, 1989, vol. 557, pp. 215-229.
Aarden et al., "Production Of Hybridoma Growth Factor By Human Monocytes", Eur. J. Immunol, #17, 1987, pp. 1411-1416.
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Revel et al., "Biological Activities Of Recombinant Human IFN-.beta.2/IL-6(E. coli)", Annals New York Academy of Sciences, vol. 557, 1987, pp. 144-156.
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Allelix Biopharmaceuticals Inc.
Gromet Nancy J.
Russel Jeffrey E.
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