Ultrahigh copy number streptomycetes plasmids

Details Ultrahigh copy number streptomycetes plasmids Ultrahigh copy number streptomycetes plasmids

1986-03-20

1990-02-06

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 43525233, 43525235, 435320, 536 27, 935 6, 935 29, 935 73, 935 75, C12N 120, C12N 1500, C07H 2104

Patent

active

048988286

ABSTRACT:
The present invention discloses an .about.1.4 kb Bc1I-Sau3A restriction fragment containing the minimal replicon of the Streptomyces coelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid derivatives constructed by in vitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyces lividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.

REFERENCES:
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