Ultrafiltration purification of glucose isomerase

Details Ultrafiltration purification of glucose isomerase Ultrafiltration purification of glucose isomerase

1984-12-20

1986-10-21

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

435814, 210651, C12N 992

Patent

active

046185844

ABSTRACT:
This invention relates to a process for the production of a purified glucose isomerase enzyme which comprises contacting an enzyme extract containing glucose isomerase and impurities with a first polysulfone membrane not normally permeable to glucose isomerase, in the presence of a salt concentration capable of selectively inducing permeation of glucose isomerase through the membrane, and obtaining a glucose isomerase containing permeate.

REFERENCES:
patent: 4522725 (1985-06-01), Koyama et al.
Method in Enzymology, vol. 22, pp. 39-49 (1971).

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