Ultra-sensitive magnetoreduction measurement system and...

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic

Reexamination Certificate

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C436S518000

Reexamination Certificate

active

08034634

ABSTRACT:
An ultra-sensitive, wash-free method for quantitatively determining the concentration of biomolecules in a sample solution is provided. The sensitivity of the method is 1 ppt or below. The method includes providing a reagent containing magnetic nanoparticles and measuring the ac magnetic susceptibility of the reagent. Then, a sample solution containing either multiple-active epitope biomolecules or single-active-epitope biomolecules is mixed with the reagent. The ac magnetic susceptibility of the reagent after the association with the biomolecules is measured. Thereafter, the difference in the ac magnetic susceptibility of the reagent before and after the association with the biomolecules is measured and the concentration of biomolecules in the sample solution is determined.

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