Ultra-high specificity fluorescent labeling

Details Ultra-high specificity fluorescent labeling Ultra-high specificity fluorescent labeling

2007-10-16

2007-10-16

Le, Long V. (Department: 1641)

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

C436S544000, C436S172000, C436S164000, C436S800000, C436S805000, C435S007100, C435S968000, C435S069700, C530S402000, C530S408000, C548S102000, C548S402000

Reexamination Certificate

active

11257292

ABSTRACT:
A self-assembled relay probe for detecting a target material is provided including: a first peptide tag bound to the target material; and a first fluorescent conjugate including a first fluorochrome and a first tag binding group; wherein the first fluorescent conjugate selectively associates with the first tag. The probe further includes a second peptide tag bound to the target material; and a second fluorescent conjugate including a second fluorochrome having a longer wavelength and distinct excitation and emission maxima from the first fluorochrome and a second tag binding group. Upon exposure to the target material, the first and second fluorescent conjugates independently associate with the first and second peptide tags, respectively, so as to be a distance apart represented by about 0.1 times R0to about 2 times R0, such that upon excitation of the first fluorescent conjugate, fluorescence resonance energy transfer results in excitation of the second fluorescent conjugate, yielding detectable emission from the second fluorescent conjugate.

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