Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2005-12-22
2008-11-11
Pak, Yong D (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S004000, C435S006120, C435S069100, C435S071100, C435S252300, C435S320100, C435S072000, C536S023200
Reexamination Certificate
active
07449316
ABSTRACT:
A novel gene defining a novel enzyme UDP-galactose: β-D-galactose-R 4-α-D-galactosyltransferase, termed α4Gal-T1, with unique enzymatic properties is disclosed. The invention provides isolated DNA molecules and DNA constructs encoding α4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting α4Gal-T1 activity, as well as cloning and expression vectors including such DNA, host cells comprising DNA encoding α4Gal-T1, and recombinant methods for providing α4Gal-T1. The enzyme α4Gal-T1 and α4Gal-active derivatives thereof are disclosed. Further, the invention discloses methods of obtaining α1, 4galactosyl glycosylated glycosphingolipids by use of an enzymatically active α4Gal-T1 protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active α4Gal-T1 protein as an expression system for recombinant production of such glycosphingolipids. Also a method for the identification of DNA sequence variations in the α4Gal-T1-coding exon by PCR, and detecting the presence of DNA sequence variation, are disclosed.
REFERENCES:
patent: 7115404 (2006-10-01), Clausen et al.
patent: WO 96 10086 (1996-04-01), None
patent: WO 01 57190 (2001-08-01), None
Almeida, R., et al., “A Family of Human β4-Galactosyltransferases: Cloning and expression of two novel UDP-Galactose: β-N-Acetylglucosamine β1, 4-Galactosyltransferases, β4Gal-T2 and β4Gal-T3”,J. Biol. Chem.,272:51:31979-31992, 1997.
Amado, M., et al., “Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions”,Biochim. Biophys. Acta in press,pp. 35-53, 1999.
Bailly, P., et al., “P Blood Group and Related Antigens”, Blood Cell Biochemistry,P. Plenum Press,,pp. 299-329, 1995.
Bailly, P., et al., “Biosynthesis of the blood group Pkand P1antigens by human kidney microsomes”,Carbohydrate Res.,228:277-287, 1992.
Bennett, E.P., et al., “cDNA Cloning and Expression of a Novel Human UDP-N-acetyl-α-D-galactrosamine”, J. Biol. Chem. 271:29:17006-17012, 1996.
Bennett, E.P., et al., “Cloning of a Human UDP-N-Acetyl-α-D-Galactosamine: Polypeptide-N-Acetylgalactosaminyltransferase That Complements Other GalNAc-Transferases in CompleteO-Glycosylation of the MUC1 Tandem Repeat”, J. Biol. Chem. 273:46:30472-30481, 1998.
Brew, K., et al., “The Role of α-Lactalbumin and the A Protein in Lactose Synthetase: A Unique Mechanism for the Control of a Biological Reaction”Proc. Natl. Acad. Sci.USA, 59:491-497, 1968.
Brodbeck, U., et al., The Isolation and Identification of the B Protein of Lactose Synthetase as “−Lactalbumin”,J. Biol. Chem.,242:7:1391-1397, 1967.
Cedergren, B., “Population studies in northern Sweden IV. Frequency of the blood type p”,Hereditas73:27-30, 1973.
Charron, M., et al., “The increased level of β1,4-galactosyltransferase required for lactose biosynthesis is achieved in part by translational control”,Proc. Natl. Sci. USA, 95:14805-14810, 1998.
Clausen, H., et al., “ABH and Related Histo-Blood Group Antigens; Immunochemical Differences in Carrier Isotypes as Their Distribution”,Vox Sang.56:1-20, 1989.
Dabrowski, J., et al., “Structural Analysis of Glycosphingolipids by High-Resolution1H Nuclear Magnetic Resonance Spectroscopy”,American Chem. Soc.,19:5652-5658, 1980.
Daniels, G.L., et al., “Terminology for Red Cell Surface Antigens”, ISBT Working Party Oslo Report, International Society of Blood Transfusion,Vox Sang.,77:52-57, 1997.
Do, Ki-Young, et al., “α-Lactalbumin Induces Bovine Milk β-1,4-Galactosyltransferase to Utilize UDP-GalNAc”,J. Biol. Chem.,270:18447-18451, 1995.
Fletcher, K.S., “P Blood Group Regulation of Glycosphingolipid Levels in Human Erythrocytes”,J. Biol. Chem.,254:22:11196-11198, 1979.
Gotschlich, E.C., “Genetic Locus for the Biosynthesis of the Variable Portion ofNeisseria genorrhoeaeLipooligosaccharide”,J. Exp. Med.,180:2181-2190, 1994.
Iizuka, S, et al., “Studies on the Human Blood Group P System: An existence of UDP-Gal:Lactosylceramide α→1,4 Galactosyltransferase in the Small ρ type cells1”, Biochem. Biophys. Res. Commun.,37:1187-1195, 1986.
Kannagi, R., et al., “New Globoseries Glycosphingolipids in Human Teratocarcinoma Reactive with the Monoclonal Antibody Directed to a Developmentally Regulated Antigen, Stage-specific Embryonic Antigen 3”,J. Biol. Chem.,258:8934-8942, 1983.
Karlsson, K.A., “Meaning and therapeutic potential of microbial recognition of host glycoconjugates”,Molecular Microbiology29(1):1-11, 1998.
Kelly, R.J., Ernst, L.K., Larsen, R.D., Bryant, J.G., Robnson, J.S. and Lowe, J.B., Molecular Basis for H blood group deficiency in Bombay (oh) and para-Bombay individuals.Proc Natl. Acad. Sci.,U.S.A. 91:5843-5847, 1994.
Kelly R.J., et al., “Sequence and expression of a candidate for the human Secretor blood group alpha (1,2) fucosyltransferase gene (FUT2). Homozygosity for an enzyme-inactivating nonsense mutation commonly correlates with the non-secretor phenotype”,J. Biol. Chem.270:4640-4649, 1995.
Keusch, J., et al., “Cloning of Gb3Synthase, the Key Enzyme in Globo-series Glycosphingolipid Synthesis, Predicts a Family of α1,4-Glycosyltransferases Conserved in Plants, Insects, and Mammals”, J. Biol. Chem., 275:33:25315-25321, Aug. 2000.
Kojima, Yoshinao, et al., “Molecular Cloning of Globotriaosylceramide/CD77 Synthase, a Glycosyltransferase That Initiates the Synthesis of Globo Series Glycosphingolipids”, J. Biol. Chem. 275:20:15152-15156, XP002901746, (May 2000).
Kozak, M., Structural features in eukaryotic mRNAs that modulate the initiation of translation,J. Biol. Chem.266:19867-19870, 1991.
Landsteiner, K., et al., “Further Observations on Individual Differences of Human Blood”,Proc. Soc. Biol. Exp. Biol. N. Y.,24:941-942.
Lopez, M., et al., “Characterization of a UDP-Gal:Galβ1-3GalNAc α1,4-Galactosyltransferase Activity in aMamestra brassicaeCell Line”,J. Biol. Chem.273:50:33644-33651, 1998.
Mandell, U., et al., “Expression of polypeptide GalNAc-transferases in stratified epithelia and squamous cell carcinomas: immunohistological evaluation using monoclonal antibodies to three members of the GalNAc-transferase family”,Glycobiology,9:1:43-52, 1999.
Mangeney, M., et al., “Apoptosis induced in Burkitt's lymphoma cells via gb3/CD77, a glycolipid antigen”,Cancer Res.53:5314-5319, 1993.
Marcus, D.M., et al., “The Ii and P Blood Group Systems”,Immunol. Ser.,43:701-712, 1989.
Marcus, D.M., et al., “Immunochemistry of the P Blood Group System”,Prog. Clin. Bliol. Res.,43:55-65, 1980.
Martin, S.L., et al., “Lewis X Biosynthesis in Helicobacter pylori”,J. Biol. Chem.,272:34:21349-21356, 1997.
McAlpine, P.J, et al., “Is the DIA1locus linked to the P blood group locus? Cytogenet”,Cell Genet.22:629-632, 1978.
Mollicone, R., et al., Molecular Basis for Lewis α (1,3/1,4)-Fucosyltransferase Gene Deficiency (FUT3) Found in Lewis-negative Indonesian Pedigrees,J. Biol. Chem.,269:33:20987-20994, 1994.
Naiki, M., et al., “Structure of the Human Erythrocyte Blood Group P1glycosphingolipid”, Biochemistry 14:4831-4837, 1975.
Naiki, M., et al., “The Human Erythricyte P and PkBlood Group Antigens: Identification as Glycosphingolipids”,Biochem. Biophys. Res. Commn.,60:1105-1111, 1974.
Nakayama, J., et al., “Expression cloning of a human α1,4-N-acetylglucosaminyltransferase that forms GlcNαc1→6Galβ→R, a glycan specifically expressed in the gastric gland mucous cell-type mucin”,Proc. Natll. Acad. Sci.,96:8991-
Bennett Eric Paul
Clausen Henrik
Steffensen Rudi
Darby & Darby P.C.
Glycozym APS
Pak Yong D
LandOfFree
UDP-galactose: β-D-galactose-R... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with UDP-galactose: β-D-galactose-R..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and UDP-galactose: β-D-galactose-R... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4029922