Ubiquitin fusion protein system for protein production in plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800205, 435 691, 435 697, 4351723, 4353201, 435419, 536 234, 536 236, 935 35, 935 61, 935 67, A01H 104, C12N 500, C12N 1500

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057737053

ABSTRACT:
Ubiquitin is an abundantly expressed protein in all eukaryotic organisms. A gene for plant ubiquitin, isolated from Arabidopsis thaliana, has been used in a plant expression vector to produce a ubiquitin-protein fusion. Natural factors in the plant cleave such a fusion protein to release ubiquitin and the desired protein. It has been found that such fusions result in protein expression levels higher than those that can be obtained from expression of the protein alone.

REFERENCES:
AS Moffatt (1992) Science 255:25.
EA Sabin et al. (1989) Bio/Technology 7:705-709.

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