Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-12-17
2000-05-30
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 29, 435 4, 435 6, 435 71, 435 23, 435 691, 435 72, 435193, 530350, G01N 3353
Patent
active
06068982&
ABSTRACT:
The present invention relates to drug screening assays which provide a systematic and practical approach for the identification of candidate agents able to inhibit ubiquitin-mediated degradation of a cell-cycle regulatory protein, such as p53, p27, myc, fos, MAT.alpha.2, or cyclins. The invention further relates to novel ubiquitin-conjugating enzymes, and uses related thereto.
REFERENCES:
patent: 5384255 (1995-01-01), Ciechanover et al.
patent: 5532348 (1996-07-01), Huibregtse et al.
Adams et al. (1993) "Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library", Nature Genetics 4:373-380.
Band et al. (1991) "Loss of p53 Protein in Human Papillomarvirus Type 16 E6-Immortalized Human Mammary Epithelial Cells", J. Virol., 65(12):6671-6676-87.
Berleth et al. (1992) "Inhibition of Ubiquitin-Protein Ligase (E3) by Mono-and Bifunctional Phenylarsenoxides" J.Biol. Chem. 267(23):16403-16411.
Bissonnette et al. (1992) "Apoptotic cell death induced by c-myc is inhibited by bcl-2", Nature 359:552-554.
Chen et al (1993) "Multiple Ubiquitin-Conjugating Enzymes Participate in the In Vivo Degradation of the Yeast MAT.varies.2 Repressor", Cell 74:357-369. (1993).
Ciechanover et al. (1991) "Degradation of Nuclear Oncoproteins by the Ubiquitin System in Vitro", Proc. Natl. Acad. Sci. USA 88:139-143.
Cook et al. (1993) "Tertiary Structures of Class I Ubiquitin-Conjugating Enzymes Are Highly Conserved: Crystal Structure of Yeast Ubc4", Biochemistry 32(50):13809-13817.
Cook et al. (1192) "Structure of a Diubiquitin Conjugate and a Model for Interaction with Ubiquitin Conjugating Enzyme (E2)", J. Biol. Chem. 267(23);16467-16471.
Wilkinson (1990) Methods in Enzymology 185: 387-397.
Hershko et al. (1983) J. Biol. Chem. 258: 8206-8214.
Ciechanover et al. (1991) Proc. Natl. Acad. Sci. USA 88: 139-143.
Hochstrasser et al. (1991) Proc. Natl. Acad. Sci. USA 88: 4606-4610.
Huibregtse et al. (1993) Mol. Cell. Biol. 13: 4918-4927.
Glotzer et al. (1991) Nature 349: 132-138.
Crook et al. (1991) "Degradation of p53 Can Be Targeted by HPV E6 Sequences Distinct from Those Required for p53 Binding and Trans-Activation", Cell 67:547-556.
Dohmen et al. (1991) "The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme", Proc. Natl. Acad. Sci. 88:7351-7355.
Ellison et al. (1991) "Epitope-tagged Ubiquitin", J. Biol Chem 266(31):21150-21157.
Evan et al. (1992) "Induction fo Apoptosis in Fibroblasts by c-myc Protein", Cell 69:119-128.
Girod et al. (1993) "Homologs of the essential ubiquitin-conjugating enzymes UBC1, 4, and 5 yeast are encoded by a multigene family in Arabidopsis thaliana", The Plant Journal 3(4):545-552.
Glotzer et al. (1991) "Cyclin is degraded by the ubiquitin pathway", Nature 349:132-138.
Hartwell et al. (1992) "Defects in a Cell Cycle Checkpoint May Be Responsible for the Genomic Instability of Cancer Cells", Cell 71:543-546.
Hershko et al. (1993) "Components of Ubiquitin-Protein Ligase System", J. Biol. Chem. 258(13):8206-8214.
Hochstrasser et al. (1990) "In Vivo Degradation of a Transcriptional Regulator: The Yeast .varies.2 Repressor", Cell 61:697-708.
Hochstrasser et al. (1991) "The Short-lived MAT .varies.2 Transcriptional Regulator if Ubiquitinated in Vivo", Proc. Natl. Acad. Sci. USA 88:4606-4610.
Hoefer et al. (1991) "Purification and partial characterization of ubiquitin-activating enzyme from Saccharomyces cerevisiae", FEBS Letters 289(1):54-58.
Holloway et al. (1993) "Anaphase Is Initiated by Proteolysis Rather Than by the Inactivation of Maturation-Promoting Factor", Cell 73:1393-1402.
Huibregtse et al. (1993) "Cloning and Expression of the cDNA for E6-AP, a Protein That Mediates the Interaction of the Human Papillomavirus E6 Oncoprotein with p53", Mol Cell Biol 13(2):775-784.
Huibregtse et al. (1993) "Localization of the E6-AP Regions That Direct Human Papillomavirus E6 Binding, Association with p53, and Ubiquitination of Associated Proteins ", Mol Cell Biol 13(8):4918-4927.
Huibregtse et al. (1995) "A Family of Proteins Structurally and Functionally Related to the E6-AP Ubiquitin-protein Ligase", Proc. Natl. Acad. Sci. USA 92:2563-2567.
Huibregtse et al. (1991) "A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18", EMBO J. 10(13):4129-4135.
Hupp et al. (1992) "Regulation of the Specific DNA Binding Function of p53", Cell 71:875-886.
Jacobs et al. (1993) "Rapid Assessment of Drug Susceptibilities of Mycobacterium tuberculosis by Means of Luciferase Reporter Phages", Science 260:819-822.
Jentsch (1992) "The Ubiquitin-conjugation System", Ann Rev Genet 26:179-207.
Klemperer et al. (1989) "A Novel, Arsenite-Sensitive E2 of the Ubiquitin Pathway: Purification and Properties", Biochemistry 28:6035-6041.
Koken et al. (1991) "Structural and Functional Conservation of Two Human Homologs of the Yeast DNA Repair Gene RAD6", Proc. Natl. Acad. Sci. 88:8865-8869.
Kumar et al. (1992) "Identification of a set of genes with developmentally down-regulated expression in the mouse brain", Biochem. Biophys. Res. Comm. 185:1155-1161.
Milner et al. (1990) "p53 Is Associated with p34.sup.cdc2 In Transformed Cells", EMBO J. 9(9):2885-2889.
Munger et al. (1989) "The E6 and E7 Genes of the Human Papillomavirus Type 16 Together Are Necessary and Sufficient for Transformation of Primary Human Keratinocytes", J. Virol. 63(10):4417-4421.
Nefsky et al. (1996) "Pub1 acts as an E6-AP-like protein ubiquitin ligase in the degradation of cdc25", EMBO J. 15(16):1301-1312.
Nepveu et al. (1987) "Alternative modes of c-myc regulation in growth factor-stimulated and differentiating cells", Oncogene 1:243-250.
Rechsteiner et al. (1991) "Natural Substrates of the Ubiquitin Proteolytic Pathway", Cell 66:615-618.
Scharer et al. (1992) "Mammalian p53 can function as a transcription factor in yeast", Nucleic Acids Research 20(7):1539-1545.
Scheffner et al. (1992) "Interaction fo the Human Papillomavirus Type 16 E6 Oncoprotein with Wild-Type and Mutant Human p53 Proteins", J. Virol. 66(8):5100-5105.
Scheffner et al. (1990) "The E6 Oncoprotein Encoded by Human Papillomavirus Types 16 and 18 Promotes the Degradation of p53", Cell 63:1129-1136.
Scheffner et al. (1992) "Targeted degradation of the retinoblastoma protein by human papillomavirus E7-E6 fusion proteins", EMBO J. 11(7):2425-2431.
Scheffner et al. (1993) "The HPV-16 E6 and E6-AP Complex Functions as a Ubiquitin-Protein Ligase in the Ubiquitination of p53", Cell 75:495-505.
Seufert and Jentsch (1990) "Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins", EMBO J. 9(2):543-550.
Treier et al. (1992) "Drosophila UbcD1 encodes a highly conserved ubiquitin-conjugating enzyme involved in selective protein degradation", EMBO J. 11(1):367-372.
Tyers et al. (1992) "The Cin3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation ", EMBO J. 11(5):1773-1784.
Watanabe et al. (1989) "Human Papillomavirus Type 16 Transformation of Primary Human Embryonic Fibroblasts Requires Expression of Open Reading Frames E6 and E7", J. Virol. 63(2):965-969.
Wilkinson (1990) "Detection and Inhibition of Ubiquitin-Dependent Proteolysis", Methods in Enzymology 185:387-397.
Zhen et al. (1993) "The ubc-2 Gene of Caenorhabditis elegans Encodes a Ubiquitin-Conjugating Enzyme Involved in Selective Protein Degradation", Mol Cell Biol 13(3):1371-1377.
Berlin Vivian
Chiu Maria Isabel
Cottarel Guillaume
Damagnez Veronique
Draetta Giulio
Longton Enrique D.
Mitotix, Inc.
Wax Robert A.
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